Transport activity of rice sucrose transporters OsSUT1 and OsSUT5

Ye Sun, Anke Reinders, Kathryn R. Lafleur, Toko Mori, John M. Ward

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Expression in Xenopus oocytes and electrophysiology was used to test for transport activity of the five sucrose transporter (SUT) homologs from rice. Expression of OsSUT1 and OsSUT5 resulted in sucrose-dependent currents that were analyzed by two-electrode voltage clamping. We examined the transport kinetics, substrate specificity and pH dependence of sucrose transport and K0.5 for sucrose. OsSUT1 showed similar features to those of other type II SUTs from monocots examined previously, with a K0.5 value of 7.50 mM at pH 5.6. In contrast, OsSUT5 had a higher substrate affinity (K0.5 = 2.32 mM at pH 5.6), less substrate specificity and less pH dependence compared with all type II SUTs tested to date. Regulation of the rice SUTs, as well as ZmSUT1 from maize and HvSUT1 from barley, by reduced (GSH) and oxidized (GSSG) forms of glutathione was tested. GSSG and GSH were found to have no significant effect on the activity of sucrose transporters when expressed in Xenopus oocytes. In conclusion, differences in transport activity between OsSUT1 and OsSUT5 indicate that type II SUTs have a range of transport activities that are tuned to their function in the plant.

Original languageEnglish (US)
Pages (from-to)114-122
Number of pages9
JournalPlant and Cell Physiology
Volume51
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

Sucrose
transporters
sucrose
rice
Glutathione Disulfide
Xenopus
substrate specificity
Substrate Specificity
Oocytes
oocytes
electrophysiology
Electrophysiology
Hordeum
Liliopsida
Constriction
electrodes
Zea mays
Glutathione
glutathione
Electrodes

Keywords

  • Electrophysiology
  • Oryza sativa
  • OsSUT1
  • OsSUT5
  • Rice
  • Sucrose transporter

Cite this

Transport activity of rice sucrose transporters OsSUT1 and OsSUT5. / Sun, Ye; Reinders, Anke; Lafleur, Kathryn R.; Mori, Toko; Ward, John M.

In: Plant and Cell Physiology, Vol. 51, No. 1, 01.2010, p. 114-122.

Research output: Contribution to journalArticle

Sun, Ye ; Reinders, Anke ; Lafleur, Kathryn R. ; Mori, Toko ; Ward, John M. / Transport activity of rice sucrose transporters OsSUT1 and OsSUT5. In: Plant and Cell Physiology. 2010 ; Vol. 51, No. 1. pp. 114-122.
@article{211b6c982d5a4068ace5dfaba42699b8,
title = "Transport activity of rice sucrose transporters OsSUT1 and OsSUT5",
abstract = "Expression in Xenopus oocytes and electrophysiology was used to test for transport activity of the five sucrose transporter (SUT) homologs from rice. Expression of OsSUT1 and OsSUT5 resulted in sucrose-dependent currents that were analyzed by two-electrode voltage clamping. We examined the transport kinetics, substrate specificity and pH dependence of sucrose transport and K0.5 for sucrose. OsSUT1 showed similar features to those of other type II SUTs from monocots examined previously, with a K0.5 value of 7.50 mM at pH 5.6. In contrast, OsSUT5 had a higher substrate affinity (K0.5 = 2.32 mM at pH 5.6), less substrate specificity and less pH dependence compared with all type II SUTs tested to date. Regulation of the rice SUTs, as well as ZmSUT1 from maize and HvSUT1 from barley, by reduced (GSH) and oxidized (GSSG) forms of glutathione was tested. GSSG and GSH were found to have no significant effect on the activity of sucrose transporters when expressed in Xenopus oocytes. In conclusion, differences in transport activity between OsSUT1 and OsSUT5 indicate that type II SUTs have a range of transport activities that are tuned to their function in the plant.",
keywords = "Electrophysiology, Oryza sativa, OsSUT1, OsSUT5, Rice, Sucrose transporter",
author = "Ye Sun and Anke Reinders and Lafleur, {Kathryn R.} and Toko Mori and Ward, {John M.}",
year = "2010",
month = "1",
doi = "10.1093/pcp/pcp172",
language = "English (US)",
volume = "51",
pages = "114--122",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Transport activity of rice sucrose transporters OsSUT1 and OsSUT5

AU - Sun, Ye

AU - Reinders, Anke

AU - Lafleur, Kathryn R.

AU - Mori, Toko

AU - Ward, John M.

PY - 2010/1

Y1 - 2010/1

N2 - Expression in Xenopus oocytes and electrophysiology was used to test for transport activity of the five sucrose transporter (SUT) homologs from rice. Expression of OsSUT1 and OsSUT5 resulted in sucrose-dependent currents that were analyzed by two-electrode voltage clamping. We examined the transport kinetics, substrate specificity and pH dependence of sucrose transport and K0.5 for sucrose. OsSUT1 showed similar features to those of other type II SUTs from monocots examined previously, with a K0.5 value of 7.50 mM at pH 5.6. In contrast, OsSUT5 had a higher substrate affinity (K0.5 = 2.32 mM at pH 5.6), less substrate specificity and less pH dependence compared with all type II SUTs tested to date. Regulation of the rice SUTs, as well as ZmSUT1 from maize and HvSUT1 from barley, by reduced (GSH) and oxidized (GSSG) forms of glutathione was tested. GSSG and GSH were found to have no significant effect on the activity of sucrose transporters when expressed in Xenopus oocytes. In conclusion, differences in transport activity between OsSUT1 and OsSUT5 indicate that type II SUTs have a range of transport activities that are tuned to their function in the plant.

AB - Expression in Xenopus oocytes and electrophysiology was used to test for transport activity of the five sucrose transporter (SUT) homologs from rice. Expression of OsSUT1 and OsSUT5 resulted in sucrose-dependent currents that were analyzed by two-electrode voltage clamping. We examined the transport kinetics, substrate specificity and pH dependence of sucrose transport and K0.5 for sucrose. OsSUT1 showed similar features to those of other type II SUTs from monocots examined previously, with a K0.5 value of 7.50 mM at pH 5.6. In contrast, OsSUT5 had a higher substrate affinity (K0.5 = 2.32 mM at pH 5.6), less substrate specificity and less pH dependence compared with all type II SUTs tested to date. Regulation of the rice SUTs, as well as ZmSUT1 from maize and HvSUT1 from barley, by reduced (GSH) and oxidized (GSSG) forms of glutathione was tested. GSSG and GSH were found to have no significant effect on the activity of sucrose transporters when expressed in Xenopus oocytes. In conclusion, differences in transport activity between OsSUT1 and OsSUT5 indicate that type II SUTs have a range of transport activities that are tuned to their function in the plant.

KW - Electrophysiology

KW - Oryza sativa

KW - OsSUT1

KW - OsSUT5

KW - Rice

KW - Sucrose transporter

UR - http://www.scopus.com/inward/record.url?scp=74549184485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74549184485&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcp172

DO - 10.1093/pcp/pcp172

M3 - Article

C2 - 19965875

AN - SCOPUS:74549184485

VL - 51

SP - 114

EP - 122

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 1

ER -