Transmission of porcine reproductive and respiratory syndrome virus by fomites (boots and coveralls)

Satoshi Otake, Scott A. Dee, Kurt D. Rossow, John Deen, Soo Joo Han, Thomas W. Molitor, Carlos Pijoan

Research output: Contribution to journalArticlepeer-review

117 Scopus citations

Abstract

Objectives: To determine if porcine reproductive and respiratory syndrome virus (PRRSV) can be transmitted to susceptible pigs by fomites or people exposed to infected pigs. Methods: Ninety-six 4-week-old pigs from a PRRSV-naive source were organized into six groups individually housed in isolation rooms (four replicate trials, 24 pigs per trial). Group 1 pigs were inoculated intranasally with PRRSV strain VR-2332 (2 mL, 105 median tissue culture infective doses per mL.) On days 5, 6, and 7 post inoculation, investigators exposed to Group 1 pigs attempted to transmit PRRSV to sentinel pigs (Groups 2 to 5) by contact. After exposure to the infected pigs, an investigator entered the Group 2 room (Direct Contact group) wearing contaminated boots and coveralls and without washing hands. In contrast, investigators who entered the rooms housing Groups 3 to 5 were required to complete specific sanitation protocols, which included changing boots and coveralls and washing hands (Danish System, Group 3); changing boots and coveralls, showering, and 12 hours down time (Standard Protocol, Group 4); and changing boots and coveralls and showering, with no down time (Alternative Protocol, Group 5). Results: The PRRSV was detected on contaminated coveralls, boots, and hands of investigators who had contacted Group 1 pigs. Transmission of PRRSV occurred between Groups 1 and 2, but not between Group 1 and Group 3, 4, or 5. Implications: The PRRSV can be transmitted to susceptible pigs by contaminated fomites (boots and coveralls) and hands; however, the use of sanitation protocols appears to limit its spread.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalJournal of Swine Health and Production
Volume10
Issue number2
StatePublished - Jan 1 2002

Keywords

  • Biosecurity
  • Fomites
  • Porcine reproductive and respiratory syndrome virus
  • Swine

Fingerprint Dive into the research topics of 'Transmission of porcine reproductive and respiratory syndrome virus by fomites (boots and coveralls)'. Together they form a unique fingerprint.

Cite this