Transmission of hepatitis b virus in clinical laboratory areas

James L. Lauer, Nancy A. Vandrunen, John W. Washburn, Henry H Balfour

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


The transmission of hepatitis B virus (HBV) in clinical laboratory areas was delineated by the use of hepatitis B surface antigen (HBsAg) as presumptive evidence for the presence of the infective agent. Twenty-six (34%) of 76 environmental surfaces sampled were positive for HBsAg. The outer surfaces of blood-and serum-specimen containers had HBsAg contamination rates of 55% (six of 11) and 44% (four of nine), respectively. Subsequent handling of pipetting aids, marking devices, and other items led to their contamination and further dissemination of HBsAg. An assay instrument for complete determinations of blood cell counts was observed to splatter and drip blood during its operation. The contamination rate for environmental surfaces associated with this instrument was 15%. The data indicate that transmission of HBV in the clinical laboratory is subtle and mainly via hand contact with contaminated items during the various steps of blood processing. These data support the concept that the portal of entry of HBV is through inapparent breaks in skin and mucous membranes.

Original languageEnglish (US)
Pages (from-to)513-516
Number of pages4
JournalJournal of Infectious Diseases
Issue number4
StatePublished - Jan 1979

Bibliographical note

Funding Information:
Received for publication December 8, 1978, and in revised form March 15, 1979. This work was supported in part by U.S. Public Health Service research grant no. AM 18883. Please address requests for reprints to Dr. James L. Lauer, Department of Environmental Health and Safety, Boynton Health Service, University of Minnesota, Minneapolis, Minnesota 55455.

Copyright 2016 Elsevier B.V., All rights reserved.


Dive into the research topics of 'Transmission of hepatitis b virus in clinical laboratory areas'. Together they form a unique fingerprint.

Cite this