Abstract
A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37°C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.
Original language | English (US) |
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Pages (from-to) | 173-178 |
Number of pages | 6 |
Journal | Gene |
Volume | 36 |
Issue number | 1-2 |
DOIs | |
State | Published - 1985 |
Bibliographical note
Funding Information:This work was supportedb y a Basil O’Connor StarterR esearchG rant (No. S-415) fromth eM arch of Dimes Birth DefectsF oundationa nd by a grant from the NIH (A120385)W. e thank Eleanor Kells for typing the manuscript.P lasmid hsp-cat1 was generouslyp rovidedb y Dr. I.B. Dawid.
Keywords
- Aedes
- Drosophila
- Recombinant DNA
- heat-shock protein promoter
- polybrene
- transfection