Transgenic overexpression of γ-cytoplasmic actin protects against eccentric contraction-induced force loss in mdx mice

Kristen A. Baltgalvis, Michele A. Jaeger, Daniel P. Fitzsimons, Stanley A. Thayer, Dawn A. Lowe, James M. Ervasti

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Background: γ-cytoplasmic (γ-cyto) actin levels are elevated in dystrophin-deficient mdx mouse skeletal muscle. The purpose of this study was to determine whether further elevation of γ-cyto actin levels improve or exacerbate the dystrophic phenotype of mdx mice.Methods: We transgenically overexpressed γ-cyto actin, specifically in skeletal muscle of mdx mice (mdx-TG), and compared skeletal muscle pathology and force-generating capacity between mdx and mdx-TG mice at different ages. We investigated the mechanism by which γ-cyto actin provides protection from force loss by studying the role of calcium channels and stretch-activated channels in isolated skeletal muscles and muscle fibers. Analysis of variance or independent t-tests were used to detect statistical differences between groups.Results: Levels of γ-cyto actin in mdx-TG skeletal muscle were elevated 200-fold compared to mdx skeletal muscle and incorporated into thin filaments. Overexpression of γ-cyto actin had little effect on most parameters of mdx muscle pathology. However, γ-cyto actin provided statistically significant protection against force loss during eccentric contractions. Store-operated calcium entry across the sarcolemma did not differ between mdx fibers compared to wild-type fibers. Additionally, the omission of extracellular calcium or the addition of streptomycin to block stretch-activated channels did not improve the force-generating capacity of isolated extensor digitorum longus muscles from mdx mice during eccentric contractions.Conclusions: The data presented in this study indicate that upregulation of γ-cyto actin in dystrophic skeletal muscle can attenuate force loss during eccentric contractions and that the mechanism is independent of activation of stretch-activated channels and the accumulation of extracellular calcium.

Original languageEnglish (US)
Article number32
JournalSkeletal muscle
Volume1
Issue number1
DOIs
StatePublished - Oct 13 2011

Keywords

  • Calcium
  • Costamere
  • Dystrophin
  • Skeletal muscle injury
  • Stretch-activated channels

Fingerprint Dive into the research topics of 'Transgenic overexpression of γ-cytoplasmic actin protects against eccentric contraction-induced force loss in mdx mice'. Together they form a unique fingerprint.

Cite this