Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

Jose B. Cibelli; Stevem L. Stice; Paul J. Golueke; Jeff J. Kane; Joseph Jerry; Cathy Blackwell; F. Abel Ponce De León; James M. Robl

doi:10.1038/nbt0798-642

Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

Jose B. Cibelli, Stevem L. Stice, Paul J. Golueke, Jeff J. Kane, Joseph Jerry, Cathy Blackwell, F. Abel Ponce De León, James M. Robl

Animal Science

Research output: Contribution to journal › Article › peer-review

286 Scopus citations

Abstract

We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then dedifferentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.

Original language	English (US)
Pages (from-to)	642-646
Number of pages	5
Journal	Nature biotechnology
Volume	16
Issue number	7
DOIs	https://doi.org/10.1038/nbt0798-642
State	Published - Jul 1 1998

Keywords

Agricultural biotechnology
Nuclear transplantation

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10.1038/nbt0798-642

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abstract = "We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then dedifferentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.",

keywords = "Agricultural biotechnology, Nuclear transplantation",

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AU - Cibelli, Jose B.

AU - Stice, Stevem L.

AU - Golueke, Paul J.

AU - Kane, Jeff J.

AU - Jerry, Joseph

AU - Blackwell, Cathy

AU - Ponce De León, F. Abel

AU - Robl, James M.

PY - 1998/7/1

Y1 - 1998/7/1

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AB - We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then dedifferentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.

KW - Agricultural biotechnology

KW - Nuclear transplantation

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Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

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