Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

Jose B. Cibelli, Stevem L. Stice, Paul J. Golueke, Jeff J. Kane, Joseph Jerry, Cathy Blackwell, F. Abel Ponce De León, James M. Robl

Research output: Contribution to journalArticlepeer-review

285 Scopus citations


We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then dedifferentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.

Original languageEnglish (US)
Pages (from-to)642-646
Number of pages5
JournalNature biotechnology
Issue number7
StatePublished - Jul 1 1998


  • Agricultural biotechnology
  • Nuclear transplantation


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