Abstract
A recombinant DNA construct containing the neo gene controlled by a promoter from the Rous sarcoma virus (RSV) was microinjected into newly fertilized, dechorionated goldfish (Carassius auratus) eggs. The neo gene confers resistance to the neomycin analog drug G-418. Results of Southern blot analyses were consistent with incorporation of single or multiple copies of the gene into the genomic DNA of one examined fish. Evidence of neo mRNA in RNA dot-blot analysis indicated that the RSV promoter had initiated transcription within a piscine genome. The utility of the neo gene as a selectable marker for transgenic fish was evaluated by G-418 selection on newly hatched and juvenile fish but proved inconclusive. Reasons for the discrepancy between neo expression and G-418 selection results are discussed.
Original language | English (US) |
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Pages (from-to) | 21-33 |
Number of pages | 13 |
Journal | Aquaculture |
Volume | 85 |
Issue number | 1-4 |
DOIs | |
State | Published - Mar 1990 |
Bibliographical note
Funding Information:The authorsg ratefullya cknowledgeJa y Maher and Michael Cashmanf or technicala ssistancea, nd Lynda Keck for assistancein manuscriptp reparation. This work was supportedi n part by Minnesota Sea Grants R/A-3 (to K.S.G.) and R/A-4 (P.B.H.) Grant NA85AA-DSG136, by grantsf rom the LegislativeC ommissiono n Minnesota Resourceso f the State of Minnesota (A.J.F., K.S.G., A.R.K. and P.B.H.), by the MinnesotaA griculturalE xperi-ment Station (K.S.G. and A.R.K.). This is article 160 70o f the Minnesota AgriculturalE xperimentS tation ScientificJ ournal Article Series.