Transcriptomic analysis of staphylococcus aureus using microarray and advanced next-generation RNA-seq Technologies

Ting Lei, Aaron Becker, Yinduo Ji

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

The transcriptome has shown tremendous potential for the comprehensive investigation of gene expression profiles and transcriptional levels in comparative biology, the identification of regulatory mechanism of transcriptional regulators, and the evaluation of target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. The traditional microarray and advanced next-generation RNA sequencing technologies (RNA-seq) provide powerful and effective tools for the determination of the transcriptome of bacterial cells. In this chapter, we provide a detailed protocol for scientists who want to investigate gene expression profiles by performing microarray and/or RNA-seq analysis, including different RNA purification methods, mRNA enrichment, decontamination, cDNA synthesis, fragmentation, biotin labeling for hybridization using Affymetrix Staphylococcus aureus chips, quantitative real-time reverse transcription PCR, and RNA-seq data analysis.

Original languageEnglish (US)
Title of host publicationMethicillin-Resistant Staphylococcus Aureus (MRSA) Protocols
PublisherHumana Press Inc.
Pages213-229
Number of pages17
ISBN (Print)9781627036634
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1085
ISSN (Print)1064-3745

Keywords

  • Advanced next-generation RNA sequencing technologies (RNA-Seq)
  • Gene expression
  • Microarray analysis
  • Real-time reverse transcription (RT) polymerase chain reaction (PCR)

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    Lei, T., Becker, A., & Ji, Y. (2014). Transcriptomic analysis of staphylococcus aureus using microarray and advanced next-generation RNA-seq Technologies. In Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols (pp. 213-229). (Methods in Molecular Biology; Vol. 1085). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-664-1_13