Recently, we established and phenotypically characterized an immortalized porcine olfactory bulb neuroblast cell line, OBGF400 (1). To facilitate the future application of these cells in studies of neurological dysfunctions and neuronal pathogen interactions, a comprehensive knowledge of their genomic variability and overall gene expression capacity was pursued. Accordingly, the OBGF400 cells were subjected to karyotyping and more extensive transcriptome analyses. Cytogenetic characterization of these cells revealed a genetic mosaicism of neuronal hyperdiploidy. A direct comparison of the OBGF400 cell transcriptome pattern, generated by utilizing the Affymetrix GeneChip Porcine Genome Array, to that of a non-neural, porcine epithelial cell line facilitated the identification of 831 probe sets preferentially hybridized by the neuroblast transcripts. Subsequent functional annotation of these OBGF400 RNAs using the Database for Annotation, Visualization and Integrated Discovery 2008 enabled their allocation to the corresponding gene ontology biological process term, thereby assisting the recognition of key elements involved in the regulation of neuronal signal transduction and neurogenesis.
Bibliographical noteFunding Information:
The authors thank Dr. J. Drnevich for the statistical analysis of the microarrays and Dr. M.A. Wallig for advice on the karyotype analysis. The cytogenetic studies were carried out by D. Lewis and Dr. S.M. Gollin in the University of Pittsburgh Cancer Institute Cytogenetics Facility, supported in part by NIH grant P30CA047904 to Ronald B. Herberman. The authors also thank Dr. W.M. Schnitzlein for his editorial review of the manuscript. This material is based upon work partially supported by the Public Health Service Award No. 5R21AI61380-2 from the National Institutes of Health.
- Cytogenetic analysis
- Neuronal aneuploidy
- Porcine neuronal progenitor cell line
- Transcriptome pattern