Transcriptional regulation of Xbr-1a/Xvent-2 homeobox gene: Analysis of its promoter region

Hyun Shik Lee, Mae Ja Park, Sung Young Lee, Yoo Seok Hwang, Hyosang Lee, Dong Hyun Roh, Jong Il Kim, Jae Bong Park, Jae Yong Lee, Hsiang fu Kung, Jaebong Kim

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Xvent homeobox proteins are induced by BMP-4 signaling and have been known to mediate many BMP-4 activities as key downstream transcriptional factors. In order to investigate the regulatory mode of Xvent transcription, we isolated genomic DNA of the Xbr-1a/Xvent-2 containing the promoter region responsive to BMP-4 signaling. The cis-acting elements located within the Xbr-1a/Xvent-2 promoter and the regulation modes by BMP-4 signaling were analyzed by serial deletion and site-directed mutagenesis experiments. The upstream -235 bp of the promoter retained the full transcriptional activity and BMP-4-response when compared with the longest promoter construct. Further analysis indicated that two separated 15 bp regions contained a strong positive element and BMP-4-response element. Site-directed mutagenesis of those regions suggests that those two regions cooperate for the promoter activity and BMP-4-response. Moreover, we found that the transcription factors, Oaz and PEBP2αA, were able to elicit additive effects with BMP-4 signaling on Xbr-1a/Xvent-2 reporter activities. These results indicate that transcriptional regulation of the Xbr-1a/Xvent-2 gene occurs in a complex mode through the cooperation of various transcription factors.

Original languageEnglish (US)
Pages (from-to)815-823
Number of pages9
JournalBiochemical and Biophysical Research Communications
Issue number5
StatePublished - 2002

Bibliographical note

Funding Information:
We thank Dr. Robert J. Lechleider for Smad1 and Smad1(3SA) cDNAs; Dr. Joan Massague for Oaz and Smad1(4SA) cDNAs; and Dr. Yoshiaki Ito for PEBP2αA cDNA. This research was supported by Korea Research Foundation Grant (KRF-2001-015-FP0038)


  • BMP-4
  • Cis-acting elements
  • Dorsoventral specification
  • Smad1
  • Transcriptional regulation
  • Xbr-1a/Xvent-2
  • Xenopus laevis embryo
  • Xvent homeobox protein


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