Transcriptional regulation of the Schizosaccharomyces pombe γ-glutamylcysteine synthetase (GCS) gene was examined using the two GCS-lacZ fusion plasmids pUGCS101 and pUGCS102, which harbor 607 bp and 447 bp upstream regions, respectively. The negatively-acting sequence was located in the -607 ∼ -447 bp upstream region of the GCS gene. The upstream sequence responsible for induction by menadione (MD) and L-buthionine-(S, R)-sulfoximine (BSO) resides in the -607 ∼ -447bp region, whereas the sequence which codes for nitric oxide induction is located within the -447 bp region, measured from the translational initiation point. Carbon source-dependent regulation of the GCS gene appeared to be dependent on the nucleotide sequence within -447 bp region. The transcription factor Pap1 is involved in the induction of the GCS gene by MD and BSO, but not by nitric oxide. Induction of the GCS gene occurring due to low glucose concentration does not depend on the presence of Papl. These data imply that induction by MD and BSO may be mediated by the Pap1 binding site, probably located in the -607 ∼ -447 region, and also that the nitric oxide-mediated regulation of the S. pombe GCS gene may share a similar mechanism with its carbon-dependent induction.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Microbiology|
|State||Published - Sep 1 2004|
- Schizosaccharomyces pombe
- Transcriptional regulation
- γ-glutamylcysteine synthetase