Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana

Sowmya S. Krishnaswamy, Sanjeeva Srivastava, Mohsen Mohammadi, Muhammad H. Rahman, Michael K. Deyholos, Nat N V Kav

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Abstract

Background. Pathogenesis-related proteins belonging to group 10 (PR10) are elevated in response to biotic and abiotic stresses in plants. Previously, we have shown a drastic salinity-induced increase in the levels of ABR17, a member of the PR10 family, in pea. Furthermore, we have also demonstrated that the constitutive expression of pea ABR17 cDNA in Arabidopsis thaliana and Brassica napus enhances their germination and early seedling growth under stress. Although it has been reported that several members of the PR10 family including ABR17 possess RNase activity, the exact mechanism by which the aforementioned characteristics are conferred by ABR17 is unknown at this time. We hypothesized that a study of differences in transcriptome between wild type (WT) and ABR17 transgenic A. thaliana may shed light on this process. Results. The molecular changes brought about by the expression of pea ABR17 cDNA in A. thaliana in the presence or absence of salt stress were investigated using microarrays consisting of 70-mer oligonucleotide probes representing 23,686 Arabidopsis genes. Statistical analysis identified number of genes which were over represented among up- or down-regulated transcripts in the transgenic line. Our results highlight the important roles of many abscisic acid (ABA) and cytokinin (CK) responsive genes in ABR17 transgenic lines. Although the transcriptional changes followed a general salt response theme in both WT and transgenic seedlings under salt stress, many genes exhibited differential expression patterns when the transgenic and WT lines were compared. These genes include plant defensins, heat shock proteins, other defense related genes, and several transcriptional factors. Our microarray results for selected genes were validated using quantitative real-time PCR. Conclusion. Transcriptional analysis in ABR17 transgenic Arabidopsis plants, both under normal and saline conditions, revealed significant changes in abundance of transcripts for many stress responsive genes, as well as those related to plant growth and development. Our results also suggest that ABR17 may mediate stress tolerance through the modulation of many ABA- and CK-responsive genes and may further our understanding of the role of ABR17 in mediating plant stress responses.

Original languageEnglish (US)
Article number91
JournalBMC plant biology
Volume8
DOIs
StatePublished - Oct 13 2008

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peas
Arabidopsis thaliana
gene expression
genetically modified organisms
genes
cytokinins
salt stress
abscisic acid
Arabidopsis
oligonucleotide probes
pathogenesis-related proteins
plant stress
biotic stress
heat shock proteins
transcriptome
stress tolerance
Brassica napus
abiotic stress
seedling growth
plant development

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Krishnaswamy, S. S., Srivastava, S., Mohammadi, M., Rahman, M. H., Deyholos, M. K., & Kav, N. N. V. (2008). Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana. BMC plant biology, 8, [91]. https://doi.org/10.1186/1471-2229-8-91

Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana. / Krishnaswamy, Sowmya S.; Srivastava, Sanjeeva; Mohammadi, Mohsen; Rahman, Muhammad H.; Deyholos, Michael K.; Kav, Nat N V.

In: BMC plant biology, Vol. 8, 91, 13.10.2008.

Research output: Contribution to journalArticle

Krishnaswamy, SS, Srivastava, S, Mohammadi, M, Rahman, MH, Deyholos, MK & Kav, NNV 2008, 'Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana', BMC plant biology, vol. 8, 91. https://doi.org/10.1186/1471-2229-8-91
Krishnaswamy, Sowmya S. ; Srivastava, Sanjeeva ; Mohammadi, Mohsen ; Rahman, Muhammad H. ; Deyholos, Michael K. ; Kav, Nat N V. / Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana. In: BMC plant biology. 2008 ; Vol. 8.
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abstract = "Background. Pathogenesis-related proteins belonging to group 10 (PR10) are elevated in response to biotic and abiotic stresses in plants. Previously, we have shown a drastic salinity-induced increase in the levels of ABR17, a member of the PR10 family, in pea. Furthermore, we have also demonstrated that the constitutive expression of pea ABR17 cDNA in Arabidopsis thaliana and Brassica napus enhances their germination and early seedling growth under stress. Although it has been reported that several members of the PR10 family including ABR17 possess RNase activity, the exact mechanism by which the aforementioned characteristics are conferred by ABR17 is unknown at this time. We hypothesized that a study of differences in transcriptome between wild type (WT) and ABR17 transgenic A. thaliana may shed light on this process. Results. The molecular changes brought about by the expression of pea ABR17 cDNA in A. thaliana in the presence or absence of salt stress were investigated using microarrays consisting of 70-mer oligonucleotide probes representing 23,686 Arabidopsis genes. Statistical analysis identified number of genes which were over represented among up- or down-regulated transcripts in the transgenic line. Our results highlight the important roles of many abscisic acid (ABA) and cytokinin (CK) responsive genes in ABR17 transgenic lines. Although the transcriptional changes followed a general salt response theme in both WT and transgenic seedlings under salt stress, many genes exhibited differential expression patterns when the transgenic and WT lines were compared. These genes include plant defensins, heat shock proteins, other defense related genes, and several transcriptional factors. Our microarray results for selected genes were validated using quantitative real-time PCR. Conclusion. Transcriptional analysis in ABR17 transgenic Arabidopsis plants, both under normal and saline conditions, revealed significant changes in abundance of transcripts for many stress responsive genes, as well as those related to plant growth and development. Our results also suggest that ABR17 may mediate stress tolerance through the modulation of many ABA- and CK-responsive genes and may further our understanding of the role of ABR17 in mediating plant stress responses.",
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