Training alters the distribution of perilipin proteins in muscle following acute free fatty acid exposure

S. O. Shepherd, J. A. Strauss, Q. Wang, J. J. Dube, B. Goodpaster, D. G. Mashek, L. S. Chow

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Key points: The lipid droplet (LD)-associated perilipin (PLIN) proteins promote intramuscular triglyceride (IMTG) storage, although whether the abundance and association of the PLIN proteins with LDs is related to the diverse lipid storage in muscle between trained and sedentary individuals is unknown. We show that lipid infusion augments IMTG content in type I fibres of both trained and sedentary individuals. Most importantly, despite there being no change in PLIN protein content, lipid infusion did increase the number of LDs connected with PLIN proteins in trained individuals only. We conclude that trained individuals are able to redistribute the pre-existing pool of PLIN proteins to an expanded LD pool during lipid infusion and, via this adaptation, may support the storage of fatty acids in IMTG. Abstract: Because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote intramuscular triglyceride (IMTG) storage, we investigated the hypothesis that differential protein content of PLINs and their distribution with LDs may be linked to the diverse lipid storage in muscle between trained and sedentary individuals. Trained (n = 11) and sedentary (n = 10) subjects, matched for age, sex and body mass index, received either a 6 h lipid or glycerol infusion in the setting of a concurrent hyperinsulinaemic–euglycaemic clamp. Sequential muscle biopsies (0, 2 and 6 h) were analysed using confocal immunofluorescence microscopy for fibre type-specific IMTG content and PLIN associations with LDs. In both groups, lipid infusion increased IMTG content in type I fibres (trained: +62%, sedentary: +79%; P < 0.05) but did not affect PLIN protein content. At baseline, PLIN2 (+65%), PLIN3 (+105%) and PLIN5 (+53%; all P < 0.05) protein content was higher in trained compared to sedentary individuals. In trained individuals, lipid infusion increased the number of LDs associated with PLIN2 (+27%), PLIN3 (+73%) and PLIN5 (+40%; all P < 0.05) in type I fibres. By contrast, in sedentary individuals, lipid infusion only increased the number of LDs not associated with PLIN proteins. Acute free fatty acid elevation therefore induces a redistribution of PLIN proteins to an expanded LD pool in trained individuals only and this may be part of the mechanism that enables fatty acids to be stored in IMTG.

Original languageEnglish (US)
Pages (from-to)5587-5601
Number of pages15
JournalJournal of Physiology
Volume595
Issue number16
DOIs
StatePublished - Aug 15 2017

Fingerprint

Nonesterified Fatty Acids
Lipids
Muscles
Triglycerides
Fatty Acids
Perilipins
Fluorescence Microscopy
Confocal Microscopy
Glycerol
Body Mass Index
Biopsy
Lipid Droplets

Keywords

  • intramuscular triglyceride
  • perilipin 2
  • perilipin 3

Cite this

Training alters the distribution of perilipin proteins in muscle following acute free fatty acid exposure. / Shepherd, S. O.; Strauss, J. A.; Wang, Q.; Dube, J. J.; Goodpaster, B.; Mashek, D. G.; Chow, L. S.

In: Journal of Physiology, Vol. 595, No. 16, 15.08.2017, p. 5587-5601.

Research output: Contribution to journalArticle

Shepherd, S. O. ; Strauss, J. A. ; Wang, Q. ; Dube, J. J. ; Goodpaster, B. ; Mashek, D. G. ; Chow, L. S. / Training alters the distribution of perilipin proteins in muscle following acute free fatty acid exposure. In: Journal of Physiology. 2017 ; Vol. 595, No. 16. pp. 5587-5601.
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