We have used cell or tissue-specific promoters to express lymphocytic choriomeningitis virus (LCMV) proteins in selected cells in independent lines of transgenic mice. Upon adoptive transfers into these mice, MHC-restricted LCMV-specific cytotoxic T lymphocytes homed specifically to either the choroid plexus (SV40 promoter) or β cells of the islets of Langerhans (rat insulin promoter). The availability of promoters specific for neurons, oligodendrocytes and astrocytes makes this approach compelling for evaluating T cell trafficking into the CNS and for analyzing antigen presentation in vivo in the CNS.
Bibliographical noteFunding Information:
This is PublicationN umber7798-NP from the Department of NeuropharmacologTyh, e Scripps Research Institute,L a Jolla, CA. This work was supported in part by USPHS grants NS-12428 and AI-09484.D ale McFarlin was a friend,s cientificc ollabora-tor and associate( of MBAO) for over the last two decades.
- Central nervous system
- Cytotoxic T lymphocytes