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Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform

  • Andrei Sakai
  • , Christopher R. Deich
  • , Frank H.T. Nelissen
  • , Aafke J. Jonker
  • , Daniela M.De C. Bittencourt
  • , Christopher P. Kempes
  • , Kim S. Wise
  • , Hans A. Heus
  • , Wilhelm T.S. Huck
  • , Katarzyna P. Adamala
  • , John I. Glass

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Immunology and Microbiology