Studies of the diffusion of proteins and lipids in the plasma membrane of cells have long pointed to the presence of membrane domains. A major challenge in the field of membrane biology has been to characterize the various cellular structures and mechanisms that impede free diffusion in cell membranes and determine the consequences that membrane compartmentalization has on cellular biology. In this review, we will provide a brief summary of the classes of domains that have been characterized to date, focusing on recent efforts to identify the properties of lipid rafts in cells through measurements of protein and lipid diffusion.
Bibliographical noteFunding Information:
This work was supported by R01 GM073846 from the NIH. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIGMS or the NIH.
- Fluorescence correlation spectroscopy
- Fluorescence recovery after photobleaching
- Lateral diffusion
- Lipid raft
- Membrane microdomain
- Single particle tracking