Tracheal cartilage isolation and decellularization

Zachary Galliger, Angela Panoskaltsis-Mortari

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations


Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to harvest decellularized cartilage tissue from donor trachea using a series of chemical and enzymatic washes and incorporating the extracellular matrix in gelatin methacrylate hydrogels. This decellularized cartilage tissue is easily incorporated into a variety of hydrogels to create a cartilage tissue scaffold.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages6
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2017.


  • Cartilage
  • Decellularization
  • Extracellular matrix
  • Hydrogel
  • Trachea


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