Abstract
Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to harvest decellularized cartilage tissue from donor trachea using a series of chemical and enzymatic washes and incorporating the extracellular matrix in gelatin methacrylate hydrogels. This decellularized cartilage tissue is easily incorporated into a variety of hydrogels to create a cartilage tissue scaffold.
Original language | English (US) |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 155-160 |
Number of pages | 6 |
DOIs | |
State | Published - 2018 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 1577 |
ISSN (Print) | 1064-3745 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media New York 2017.
Keywords
- Cartilage
- Decellularization
- Extracellular matrix
- Hydrogel
- Trachea