Tracheal cartilage isolation and decellularization

Zachary Galliger, Angela Panoskaltsis-Mortari

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to harvest decellularized cartilage tissue from donor trachea using a series of chemical and enzymatic washes and incorporating the extracellular matrix in gelatin methacrylate hydrogels. This decellularized cartilage tissue is easily incorporated into a variety of hydrogels to create a cartilage tissue scaffold.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-160
Number of pages6
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1577
ISSN (Print)1064-3745

Keywords

  • Cartilage
  • Decellularization
  • Extracellular matrix
  • Hydrogel
  • Trachea

PubMed: MeSH publication types

  • Journal Article

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  • Cite this

    Galliger, Z., & Panoskaltsis-Mortari, A. (2018). Tracheal cartilage isolation and decellularization. In Methods in Molecular Biology (pp. 155-160). (Methods in Molecular Biology; Vol. 1577). Humana Press Inc.. https://doi.org/10.1007/7651_2017_52