Toxin production in soybean (Glycine max L.) plants with charcoal rot disease and by macrophomina phaseolina, the fungus that causes the disease

Hamed K. Abbas, Nacer Bellaloui, Cesare Accinelli, James R. Smith, W. Thomas Shier

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Charcoal rot disease, caused by the fungus Macrophomina phaseolina, results in major economic losses in soybean production in southern USA. M. phaseolina has been proposed to use the toxin (-)-botryodiplodin in its root infection mechanism to create a necrotic zone in root tissue through which fungal hyphae can readily enter the plant. The majority (51.4%) of M. phaseolina isolates from plants with charcoal rot disease produced a wide range of (-)-botryodiplodin concentrations in a culture medium (0.14-6.11 μg/mL), 37.8% produced traces below the limit of quantification (0.01 μg/mL), and 10.8% produced no detectable (-)-botryodiplodin. Some culture media with traces or no (-)-botryodiplodin were nevertheless strongly phytotoxic in soybean leaf disc cultures, consistent with the production of another unidentified toxin(s). Widely ranging (-)-botryodiplodin levels (traces to 3.14 μg/g) were also observed in the roots, but not in the aerial parts, of soybean plants naturally infected with charcoal rot disease. This is the first report of (-)-botryodiplodin in plant tissues naturally infected with charcoal rot disease. No phaseolinone was detected in M. phaseolina culture media or naturally infected soybean tissues. These results are consistent with (-)-botryodiplodin playing a role in the pathology of some, but not all, M. phaseolina isolates from soybeans with charcoal rot disease in southern USA.

Original languageEnglish (US)
Article number645
JournalToxins
Volume11
Issue number11
DOIs
StatePublished - Nov 6 2019

Bibliographical note

Funding Information:
Funding: This research was funded by the Mississippi Soybean Promotion Board, grant numbers 34-2016 and 34-2017.

Keywords

  • Charcoal rot disease
  • Fungi
  • Lc/MS
  • Mycotoxins
  • Phaseolinone
  • Root infection mechanism
  • Soybean

Fingerprint Dive into the research topics of 'Toxin production in soybean (Glycine max L.) plants with charcoal rot disease and by macrophomina phaseolina, the fungus that causes the disease'. Together they form a unique fingerprint.

Cite this