Toward a rapid method for the study of biodiversity in cold environments: the characterization of psychrophilic yeasts by MALDI-TOF mass spectrometry

Joseph J. Dalluge, Elizabeth C. Brown, Laurie B. Connell

Research output: Contribution to journalArticle

Abstract

To investigate the potential of matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS) as a platform to support biodiversity and phylogenetic studies of psychrophilic yeasts in cold environments, the technique was employed to rapidly characterize and distinguish three psychrophilic yeasts (Rhodotorula mucilaginosa, Naganishia vishniacii, and Dioszegia cryoxerica) from three mesophilic counterparts (Saccharomyces cerevisiae Cry Havoc, S. cerevisiae California V Ale, and S. pastorianus). A detailed workflow for providing reproducible mass spectral fingerprints of low molecular weight protein/peptide features specific to the organisms studied is presented. The potential of this approach as a tool in the study of biodiversity, systematics, and phylogeny of psychrophilic microorganisms is highlighted.

Original languageEnglish (US)
Pages (from-to)461-466
Number of pages6
JournalExtremophiles
Volume23
Issue number4
DOIs
StatePublished - Jul 1 2019

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Biodiversity
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Saccharomyces cerevisiae
Mass Spectrometry
Yeasts
Rhodotorula
Workflow
Dermatoglyphics
Phylogeny
Molecular Weight
Peptides
Proteins

Keywords

  • Antarctic
  • Arctic
  • Biodiversity
  • Fungi
  • Phylogeny
  • Systematics
  • Yeasts

PubMed: MeSH publication types

  • Journal Article

Cite this

Toward a rapid method for the study of biodiversity in cold environments : the characterization of psychrophilic yeasts by MALDI-TOF mass spectrometry. / Dalluge, Joseph J.; Brown, Elizabeth C.; Connell, Laurie B.

In: Extremophiles, Vol. 23, No. 4, 01.07.2019, p. 461-466.

Research output: Contribution to journalArticle

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