Toward a better understanding of the genomic region harboring Fusarium head blight resistance QTL Qfhs.ndsu-3AS in durum wheat

Xianwen Zhu, Shaobin Zhong, Shiaoman Chao, Yong Qiang Gu, Shahryar F. Kianian, Elias Elias, Xiwen Cai

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Key message: New molecular markers were developed and mapped to the FHB resistance QTLregion in high resolution. Micro-collinearity of the QTL region with rice andBrachypodiumwas revealed for a better understanding of the genomic region. Abstract: The wild emmer wheat (Triticum dicoccoides)-derived Fusarium head blight (FHB) resistance quantitative trait locus (QTL) Qfhs.ndsu-3AS previously mapped to the short arm of chromosome 3A (3AS) in a population of recombinant inbred chromosome lines (RICLs). This study aimed to attain a better understanding of the genomic region harboring Qfhs.ndsu-3AS and to improve the utility of the QTL in wheat breeding. Micro-collinearity of the QTL region with rice chromosome 1 and Brachypodium chromosome 2 was identified and used for marker development in saturation mapping. A total of 42 new EST-derived sequence tagged site (STS) and simple sequence repeat (SSR) markers were developed and mapped to the QTL and nearby regions on 3AS. Further comparative analysis revealed a complex collinearity of the 3AS genomic region with their collinear counterparts of rice and Brachypodium. Fine mapping of the QTL region resolved five co-segregating markers (Xwgc1186/Xwgc716/Xwgc1143/Xwgc501/Xwgc1204) into three distinct loci proximal to Xgwm2, a marker previously reported to be closely linked to the QTL. Four other markers (Xwgc1226, Xwgc510, Xwgc1296, and Xwgc1301) mapped farther proximal to the above markers in the QTL region with a higher resolution. Five homozygous recombinants with shortened T. dicoccoides chromosomal segments in the QTL region were recovered by molecular marker analysis and evaluated for FHB resistance. Qfhs.ndsu-3AS was positioned to a 5.2 cM interval flanked by the marker Xwgc501 and Xwgc510. The recombinants containing Qfhs.ndsu-3AS and new markers defining the QTL will facilitate utilization of this resistance source in wheat breeding.

Original languageEnglish (US)
Pages (from-to)31-43
Number of pages13
JournalTheoretical and Applied Genetics
Volume129
Issue number1
DOIs
StatePublished - Jan 1 2016

Bibliographical note

Funding Information:
The authors would like to acknowledge all members in Cai?s lab and Dr. Chenggen Chu for their help in this research. Also, we thank Drs. Lili Qi and Rebekah Oliver for their critical review of the manuscript. This material is based upon work supported by the US Department of Agriculture, under Agreement No. 59-0790-8-068. This is a cooperative project with the US Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture.

Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.

Fingerprint Dive into the research topics of 'Toward a better understanding of the genomic region harboring Fusarium head blight resistance QTL Qfhs.ndsu-3AS in durum wheat'. Together they form a unique fingerprint.

Cite this