Total synthesis and evaluation of C25-benzyloxyepothilone C for tubulin assembly and cytotoxicity against MCF-7 breast cancer cells

Oliver E. Hutt, Bollu S. Reddy, Sajiv K. Nair, Emily A. Reiff, John T. Henri, Jack F. Greiner, Ting Lan Chiu, David G. VanderVelde, Elizabeth A. Amin, Richard H. Himes, Gunda I. Georg

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The total synthesis of C25-benzyloxy epothilone C is described. A sequential Suzuki-Aldol-Yamaguchi macrolactonization strategy was utilized employing a novel derivatized C8-C12 fragment. The C25-benzyloxy analog exhibited significantly reduced biological activity in microtubule assembly and cytotoxicity assays. Molecular modeling simulations indicated that excessive steric bulk in the C25 position may reduce activity by disrupting key hydrogen bonds that are crucial for epothilone binding to β-tubulin.

Original languageEnglish (US)
Pages (from-to)4904-4906
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume18
Issue number17
DOIs
StatePublished - Sep 1 2008

Keywords

  • C-25 epothilone analogue
  • Cytotoxicity
  • Epothilones
  • Total synthesis
  • Tubulin assembly

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