Toluene 2-monooxygenase-dependent growth of Burkholderia cepacia G4/PR1 on diethyl ether

Hor Gil Hur, Lisa M. Newman, Lawrence P. Wackett, Michael J. Sadowsky

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Aerobic bacterial growth on aromatic hydrocarbons typically requires oxygenase enzymes, which are known to fortuitously oxidize nongrowth substrates. In this study, we found that oxidation of diethyl ether by toluene 2-monooxygenase supported more rapid growth of Burkholderia cepacia G4/PR1 than did the aromatic substrates n-propylbenzene and o-xylene. The wild-type Burkholderia cepacia G4 failed to grow on diethyl ether. Purified toluene 2-monooxygenase protein components oxidized diethyl ether stoichiometrically to ethanol and acetaldehyde. Butyl methyl ether, diethyl sulfide, and 2-chloroethyl ethyl ether were oxidized by B. cepacia G4/PR1.

Original languageEnglish (US)
Pages (from-to)1606-1609
Number of pages4
JournalApplied and environmental microbiology
Volume63
Issue number4
DOIs
StatePublished - Apr 1997

Fingerprint Dive into the research topics of 'Toluene 2-monooxygenase-dependent growth of Burkholderia cepacia G4/PR1 on diethyl ether'. Together they form a unique fingerprint.

Cite this