TY - JOUR
T1 - Toluene 2-monooxygenase-dependent growth of Burkholderia cepacia G4/PR1 on diethyl ether
AU - Hur, Hor Gil
AU - Newman, Lisa M.
AU - Wackett, Lawrence P.
AU - Sadowsky, Michael J.
PY - 1997/4
Y1 - 1997/4
N2 - Aerobic bacterial growth on aromatic hydrocarbons typically requires oxygenase enzymes, which are known to fortuitously oxidize nongrowth substrates. In this study, we found that oxidation of diethyl ether by toluene 2-monooxygenase supported more rapid growth of Burkholderia cepacia G4/PR1 than did the aromatic substrates n-propylbenzene and o-xylene. The wild-type Burkholderia cepacia G4 failed to grow on diethyl ether. Purified toluene 2-monooxygenase protein components oxidized diethyl ether stoichiometrically to ethanol and acetaldehyde. Butyl methyl ether, diethyl sulfide, and 2-chloroethyl ethyl ether were oxidized by B. cepacia G4/PR1.
AB - Aerobic bacterial growth on aromatic hydrocarbons typically requires oxygenase enzymes, which are known to fortuitously oxidize nongrowth substrates. In this study, we found that oxidation of diethyl ether by toluene 2-monooxygenase supported more rapid growth of Burkholderia cepacia G4/PR1 than did the aromatic substrates n-propylbenzene and o-xylene. The wild-type Burkholderia cepacia G4 failed to grow on diethyl ether. Purified toluene 2-monooxygenase protein components oxidized diethyl ether stoichiometrically to ethanol and acetaldehyde. Butyl methyl ether, diethyl sulfide, and 2-chloroethyl ethyl ether were oxidized by B. cepacia G4/PR1.
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U2 - 10.1128/aem.63.4.1606-1609.1997
DO - 10.1128/aem.63.4.1606-1609.1997
M3 - Article
C2 - 16535583
AN - SCOPUS:0030907727
SN - 0099-2240
VL - 63
SP - 1606
EP - 1609
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 4
ER -