Tolerance induction by third-party "off-the-shelf" CD4 +CD25+ Treg cells

David Steiner, Noga Brunicki, Bruce R. Blazar, Esther Bachar-Lustig, Yair Reisner

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Objective. Recent reports have shown that donor or host CD4 +CD25+ Treg cells can be used to control GVHD or graft rejection following allogeneic BMT in mice. In the present study we investigated the potential of third-party Treg cells compared to donor-type cells to facilitate BM allografting. Methods. Graft rejection is assessed in a mouse model of T cell-mediated BM allograft rejection. Lethally irradiated C3H mice are transplanted at day 2 after irradiation with T cell-depleted Balb/Nude BM. Graft rejection is induced by purified host-type T cells infused one day prior to BMT. Cells tested for their facilitating activity are added to the T cell-depleted BM allograft. Results. Naïve or ex vivo-expanded third-party Treg cells can effectively enhance engraftment of T cell-depleted BM allografts, exhibiting reactivity in vitro and in vivo similar to that found for donor-type Treg cells. Conclusion. The use of third-party Treg cells in contrast to donor-type cells could allow advanced preparation of a large bank of Treg cells (off-the-shelf), with all the appropriate quality controls required for cell therapy.

Original languageEnglish (US)
Pages (from-to)66-71
Number of pages6
JournalExperimental Hematology
Volume34
Issue number1
DOIs
StatePublished - Jan 1 2006

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