TNFα-p55 receptors: Medullary brainstem immunocytochemical localization in normal and vagus nerve-transected rats

Gerlinda E. Hermann, Sadie L. Hebert, Montina J. Van Meter, Gregory M. Holmes, Richard C. Rogers

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Tumor necrosis factor alpha (TNFα) is a potent modulator of autonomic reflex mechanisms that control the stomach. Evidence suggests that TNFα action directly on vago-vagal reflex control circuits causes the autonomic misregulation of digestion manifested as gastrointestinal stasis, nausea, and emesis associated with illness. Neurophysiological studies indicated that TNFα may have effects on vagal afferents in the solitary nucleus, as well as neurons of the solitary nucleus (NST) and dorsal motor nucleus (DMN) of the vagus. The aim of this study was to determine the location of the TNFR1 receptor (p55) in the medulla using immunocytochemical methods. We devised a technique for localizing the p55 receptor using heat-induced antigen recovery in fixed tissue sections. This protocol allowed us to demonstrate that dense p55-immunoreactivity (p55-ir) is constitutively present on central (but not peripheral) vagal afferents in the solitary tract (ST) and nucleus; p55-ir is also present on afferents entering the spinal trigeminal nucleus. Unilateral supra-nodose vagotomy eliminated p55-ir from ipsilateral central vagal afferents. Virtually all neurons in the brainstem appeared to express p55-ir at a low level, i.e., just above background. However, vagotomy caused a dramatic up-regulation of p55-ir in vagal motor neurons. This increase in p55-ir in axotomized neurons may play a pivotal role in the connection between the occurrence of the injury and the initiation of apoptotic processes resulting in elimination of damaged neurons.

Original languageEnglish (US)
Pages (from-to)156-166
Number of pages11
JournalBrain Research
Volume1004
Issue number1-2
DOIs
StatePublished - Apr 9 2004

Fingerprint

Vagus Nerve
Brain Stem
Solitary Nucleus
Tumor Necrosis Factor-alpha
Neurons
Vagotomy
Reflex
Spinal Trigeminal Nucleus
Receptors, Tumor Necrosis Factor, Type I
Motor Neurons
Nausea
Vomiting
Digestion
Stomach
Up-Regulation
Hot Temperature
Antigens
Wounds and Injuries

Keywords

  • Apoptosis
  • Cytokine
  • Endocrine and autonomic regulation
  • Immune-neural interaction
  • Nerve injury
  • Neural-immune interactions
  • TNFR1
  • Wallerian degeneration

Cite this

TNFα-p55 receptors : Medullary brainstem immunocytochemical localization in normal and vagus nerve-transected rats. / Hermann, Gerlinda E.; Hebert, Sadie L.; Van Meter, Montina J.; Holmes, Gregory M.; Rogers, Richard C.

In: Brain Research, Vol. 1004, No. 1-2, 09.04.2004, p. 156-166.

Research output: Contribution to journalArticle

Hermann, Gerlinda E. ; Hebert, Sadie L. ; Van Meter, Montina J. ; Holmes, Gregory M. ; Rogers, Richard C. / TNFα-p55 receptors : Medullary brainstem immunocytochemical localization in normal and vagus nerve-transected rats. In: Brain Research. 2004 ; Vol. 1004, No. 1-2. pp. 156-166.
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