Tissue specificity of the sugarcane bacilliform virus promoter in oat, barley and wheat

Nadiya A. Al-Saady, Kimberly A. Torbert, Lisa Smith, Irina Makarevitch, Gerald Baldridge, Richard J. Zeyen, Gary J. Muehlbauer, Neil E. Olszewski, David A. Somers

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The tissue-specificity of the sugarcane bacilliform virus SCBV promoter was investigated in oat, barley, and wheat to determine whether its expression pattern in one species was predictive of promoter specificity in the other closely related Gramineae species. Progeny of transgenic plants produced using constructs containing the SCBV promoter driving gusA were sampled at different stages of plant development and stained for GUS activity using a histochemical assay. Overall, the GUS staining patterns were most similar between oat and barley. In all three species, similar GUS staining patterns were observed in mature endosperms, leaves, and floral bracts of developing infloresences. No GUS staining was detected in oat embryos whereas the entire barley embryo was stained, and GUS staining was confined to the scutellum of wheat embryos. Oat and barley stems exhibited GUS staining whereas no GUS staining was observed in stems of the transgenic wheat plants. The SCBV promoter conferred strong GUS staining intensity in most tissues of oat and barley but was generally weaker in wheat. These differences in SCBV promoter specificity indicate that promoter evaluation should be conducted in the target species of interest rather than by extrapolation from expression patterns in other species.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalMolecular Breeding
Volume14
Issue number3
DOIs
StatePublished - Oct 1 2004

Keywords

  • Microprojectile bombardment
  • Tissue-specific expression
  • Transgene
  • Triticeae
  • β-glucuronidase

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