Tissue specific expression of p422 protein, a putative lipid carrier, in mouse adipocytes

David A. Bernlohr, Tamara L. Doering, Thomas J. Kelly, M. Daniel Lane

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The differentiation of 3T3-L1 preadipocytes leads to the expression of a new protein, p422, and its mRNA. This protein has 70% and 20-30% amino acid sequence homology to myelin P2 and the fatty acid binding proteins of liver and intestine, respectively. Investigation of the distribution in mouse tissues of p422 protein by immunoblotting and of p422 mRNA by cDNA hybridization indicates that they are expressed only in adipose tissue. Liver and intestinal fatty acid binding protein mRNA's were not detectable in mouse adipose tissue or in 3T3-L1 adipocytes. It is suggested that p422 functions as an adipocyte fatty acid binding protein.

Original languageEnglish (US)
Pages (from-to)850-855
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Oct 30 1985

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS We wish to thank Dr. Bruce Trapp for providing the bovine anti-myelin P2 antiserum and Drs. L. Chan and L. Smith for providing the rat liver fatty acid binding protein cDNA. We are indebted to Dr. Fred Bollum for communicating the immunoblot procedure to us and to Dr. Susan Buhrow and Eric Sibley for aid in preparation of tissue extracts. The technical assistance of Margit Lucskay is gratefully acknowledged as is Norma Mitchell for preparing this manuscript. D.A.B. was supported by NIH Research Service Award 1 F32 AM06954 (Current address: Department of Biochemistry, University of Minnesota), T.L.D. by NIH Medical Scientist Training Program Award 2T32GM07309, T.J.K. 5 PO1 CA16519, and M.D.L by NIH grant AM14574.


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