Tissue-specific expression of novel messenger ribonucleic acids cloned from a renin-expressing kidney tumor cell line (as4.1)

Heather A. Thompsons, John M. Bursonf, Julie A. Lang, Kenneth W. Gross, Curt D. Sigmund

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

As4.1 cells are derived from a renin-expressing kidney tumor induced by tissue-specific oncogene-mediated tumorigenesis in transgenic mice. These cells express high levels of renin messenger RNA (mRNA) and synthesize prorenin and renin; they were therefore used as a model to further investigate the molecular biology of renin-producing kidney cells by cloning and characterizing novel mRNAs expressed in these cells. One clone, designated 1.5, was randomly selected from an As4.1 complementary DNA (cDNA) library, and two other cDNA clones, designated 4.9 and 6.9, were obtained by screening the cDNA library using a strategy to identify As4.1 cell-specific mRNAs. Each clone exhibited a highly restricted tissue-specific expression profile, including high level expression in As4.1 cells and low level expression in kidney. No homology was found between the sequence of the partial 1.5 and 4.9 cDNAs and sequences in Genbank. Southern blot analysis revealed that clone 4.9 is encoded by a single copy gene containing at least two separate exons. A homology search of the sequence of clone 6.9 revealed it to encode a cDNA to serum amyloid A protein; consistent with this identification, expression of 6.9 mRNA was highly induced in both kidney and liver after treatment of mice with Escherichia coli lipopolysaccharide.

Original languageEnglish (US)
Pages (from-to)3037-3045
Number of pages9
JournalEndocrinology
Volume136
Issue number7
DOIs
StatePublished - Jul 1995

Fingerprint Dive into the research topics of 'Tissue-specific expression of novel messenger ribonucleic acids cloned from a renin-expressing kidney tumor cell line (as4.1)'. Together they form a unique fingerprint.

Cite this