Tissue sources of protein kinase activities in human seminal fluid: Studies of normal, oligozoospermic, and vasectomized men

M. J. Wilson; K. W. Kaye

doi:10.1016/s0015-0282(16)47186-4

Tissue sources of protein kinase activities in human seminal fluid: Studies of normal, oligozoospermic, and vasectomized men

M. J. Wilson
, K. W. Kaye

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Protein kinase activities in seminal fluids of normo-, hypo-, and oligozoospermic and vasectomized men were measured using lysine-rich histones and partially dephosphorylated phosvitin as acceptor substrates. There was a significant relationship of histone kinase but not phosvitin kinase activities with the number of spermatozoa originally present in the semen. Histone kinase and phosvitin kinase activities were diminished 88% and 62%, respectively, in vasectomy seminal fluid. The sex accessory gland sources of seminal fluid protein kinase activities not associated with spermatozoa were examined in split ejaculates of vasectomized men. Histone kinase activity was greater in the first fractions, suggesting that the prostate is its predominant contributor; whereas the distribution of phosvitin kinase activity did not indicate any preferential accessory gland source of this enzyme.

Original language	English (US)
Pages (from-to)	105-109
Number of pages	5
Journal	Fertility and Sterility
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/s0015-0282(16)47186-4
State	Published - 1983

Bibliographical note

Funding Information:
Received August 23, 1982; revised and accepted March 2, 1983. *Supported in part by the General Medical Research Funds of the Veterans Administration and the Minnesota Medical Foundation grant CRF~48-81. tToxicology Research Laboratory, Veterans Administration Medical Center, and Department of Laboratory Medicine and Pathology, University of Minnesota. tReprint requests: Dr. Michael J. Wilson, Toxicology Research Laboratory, Veterans Administration Medical Center, Minneapolis, Minnesota 55417. §Department of Surgery Service, Veterans Administration Medical Center, and Urologic Surgery, University of Minnesota.

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title = "Tissue sources of protein kinase activities in human seminal fluid: Studies of normal, oligozoospermic, and vasectomized men",

abstract = "Protein kinase activities in seminal fluids of normo-, hypo-, and oligozoospermic and vasectomized men were measured using lysine-rich histones and partially dephosphorylated phosvitin as acceptor substrates. There was a significant relationship of histone kinase but not phosvitin kinase activities with the number of spermatozoa originally present in the semen. Histone kinase and phosvitin kinase activities were diminished 88\% and 62\%, respectively, in vasectomy seminal fluid. The sex accessory gland sources of seminal fluid protein kinase activities not associated with spermatozoa were examined in split ejaculates of vasectomized men. Histone kinase activity was greater in the first fractions, suggesting that the prostate is its predominant contributor; whereas the distribution of phosvitin kinase activity did not indicate any preferential accessory gland source of this enzyme.",

author = "Wilson, \{M. J.\} and Kaye, \{K. W.\}",

note = "Funding Information: Received August 23, 1982; revised and accepted March 2, 1983. *Supported in part by the General Medical Research Funds of the Veterans Administration and the Minnesota Medical Foundation grant CRF\textasciitilde{}48-81. tToxicology Research Laboratory, Veterans Administration Medical Center, and Department of Laboratory Medicine and Pathology, University of Minnesota. tReprint requests: Dr. Michael J. Wilson, Toxicology Research Laboratory, Veterans Administration Medical Center, Minneapolis, Minnesota 55417. §Department of Surgery Service, Veterans Administration Medical Center, and Urologic Surgery, University of Minnesota.",

year = "1983",

doi = "10.1016/s0015-0282(16)47186-4",

language = "English (US)",

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pages = "105--109",

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T1 - Tissue sources of protein kinase activities in human seminal fluid

T2 - Studies of normal, oligozoospermic, and vasectomized men

AU - Wilson, M. J.

AU - Kaye, K. W.

N1 - Funding Information: Received August 23, 1982; revised and accepted March 2, 1983. *Supported in part by the General Medical Research Funds of the Veterans Administration and the Minnesota Medical Foundation grant CRF~48-81. tToxicology Research Laboratory, Veterans Administration Medical Center, and Department of Laboratory Medicine and Pathology, University of Minnesota. tReprint requests: Dr. Michael J. Wilson, Toxicology Research Laboratory, Veterans Administration Medical Center, Minneapolis, Minnesota 55417. §Department of Surgery Service, Veterans Administration Medical Center, and Urologic Surgery, University of Minnesota.

PY - 1983

Y1 - 1983

N2 - Protein kinase activities in seminal fluids of normo-, hypo-, and oligozoospermic and vasectomized men were measured using lysine-rich histones and partially dephosphorylated phosvitin as acceptor substrates. There was a significant relationship of histone kinase but not phosvitin kinase activities with the number of spermatozoa originally present in the semen. Histone kinase and phosvitin kinase activities were diminished 88% and 62%, respectively, in vasectomy seminal fluid. The sex accessory gland sources of seminal fluid protein kinase activities not associated with spermatozoa were examined in split ejaculates of vasectomized men. Histone kinase activity was greater in the first fractions, suggesting that the prostate is its predominant contributor; whereas the distribution of phosvitin kinase activity did not indicate any preferential accessory gland source of this enzyme.

AB - Protein kinase activities in seminal fluids of normo-, hypo-, and oligozoospermic and vasectomized men were measured using lysine-rich histones and partially dephosphorylated phosvitin as acceptor substrates. There was a significant relationship of histone kinase but not phosvitin kinase activities with the number of spermatozoa originally present in the semen. Histone kinase and phosvitin kinase activities were diminished 88% and 62%, respectively, in vasectomy seminal fluid. The sex accessory gland sources of seminal fluid protein kinase activities not associated with spermatozoa were examined in split ejaculates of vasectomized men. Histone kinase activity was greater in the first fractions, suggesting that the prostate is its predominant contributor; whereas the distribution of phosvitin kinase activity did not indicate any preferential accessory gland source of this enzyme.

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U2 - 10.1016/s0015-0282(16)47186-4

DO - 10.1016/s0015-0282(16)47186-4

M3 - Article

C2 - 6862038

AN - SCOPUS:0020566119

SN - 0015-0282

VL - 40

SP - 105

EP - 109

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 1

ER -

Tissue sources of protein kinase activities in human seminal fluid: Studies of normal, oligozoospermic, and vasectomized men

Abstract

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