Tissue sources of protein kinase activities in human seminal fluid: Studies of normal, oligozoospermic, and vasectomized men

M. J. Wilson, K. W. Kaye

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Protein kinase activities in seminal fluids of normo-, hypo-, and oligozoospermic and vasectomized men were measured using lysine-rich histones and partially dephosphorylated phosvitin as acceptor substrates. There was a significant relationship of histone kinase but not phosvitin kinase activities with the number of spermatozoa originally present in the semen. Histone kinase and phosvitin kinase activities were diminished 88% and 62%, respectively, in vasectomy seminal fluid. The sex accessory gland sources of seminal fluid protein kinase activities not associated with spermatozoa were examined in split ejaculates of vasectomized men. Histone kinase activity was greater in the first fractions, suggesting that the prostate is its predominant contributor; whereas the distribution of phosvitin kinase activity did not indicate any preferential accessory gland source of this enzyme.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalFertility and Sterility
Volume40
Issue number1
DOIs
StatePublished - 1983

Bibliographical note

Funding Information:
Received August 23, 1982; revised and accepted March 2, 1983. *Supported in part by the General Medical Research Funds of the Veterans Administration and the Minnesota Medical Foundation grant CRF~48-81. tToxicology Research Laboratory, Veterans Administration Medical Center, and Department of Laboratory Medicine and Pathology, University of Minnesota. tReprint requests: Dr. Michael J. Wilson, Toxicology Research Laboratory, Veterans Administration Medical Center, Minneapolis, Minnesota 55417. §Department of Surgery Service, Veterans Administration Medical Center, and Urologic Surgery, University of Minnesota.

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