Tissue interactions affecting the migration and differentiation of neural crest cells in the chick embryo

C. D. Stern, K. B. Artinger, M. Bronner-Fraser

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

A series of microsurgical operations was performed in chick embryos to study the factors that control the polarity, position and differentiation of the sympathetic and dorsal root ganglion cells developing from the neural crest. The neural tube, with or without the notochord, was rotated by 180° dorsoventrally to cause the neural crest cells to emerge ventrally. In some embryos, the notochord was ablated, and in others a second notochord was implanted. Sympathetic differentiation was assessed by catecholamine fluorescence after aldehyde fixation. Neural crest cells emerging from an inverted neural tube migrate in a ventral-to-dorsal direction through the sclerotome, where they become segmented by being restricted to the rostral half of each sclerotome. Both motor axons and neural crest cells avoid the notochord and the extracellular matrix that surrounds it, but motor axons appear also to be attracted to the notochord until they reach its immediate vicinity. The dorsal root ganglia always form adjacent to the neural tube and their dorsoventral orientation follows the direction of migration of the neural crest cells. Differentiation of catecholaminergic cells only occurs near the aorta/mesonephros and in addition requires the proximity of either the ventral neural tube (floor plate/ventral root region) or the notochord. Prior migration of presumptive catecholaminergic cells through the sclerotome, however, is neither required nor sufficient for their adrenergic differentiation.

Original languageEnglish (US)
Pages (from-to)207-216
Number of pages10
JournalDevelopment
Volume113
Issue number1
StatePublished - 1991
Externally publishedYes

Keywords

  • Catecholamines Adrenergic neurons
  • Chick embryo
  • Differentiation
  • Neural crest
  • Segmentation
  • Sympathetic nervous system

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