The octamer motif is important in transcriptional regulation of genes of the immune system in many species, including the chicken. Little is known, however, regarding octamer-binding protein expression in chicken tissues. We examined octamer-binding protein expression patterns in multiple chicken tissues (ovary, cerebrum, liver, lung, kidney, spleen, thymus, and bursa of Fabricius) plus two lymphocyte cell lines. Every tissue and cell line had multiple octamer-binding proteins. Seven distinct protein-DNA complexes were identified. Our results demonstrate that multiple octamer-binding proteins, exhibiting differential tissue expression, exist in a wide variety of tissues of the chicken.
Bibliographical noteFunding Information:
We thank Tammy Nowling, Iowa State University for technical advice and mouse Day 12.5 and Day 15 embryo nuclear extracts; Craig Thompson, University of Chicago, for the DT40 and MSB1 cell lines. This work was partly supported by the USDA NRICGP grant 95-37205-2191, Hatch, and state of Iowa funds.
- Differential tissue expression
- Octamer motif
- Transcriptional regulation