Tissue Contraction Force Microscopy for Optimization of Engineered Cardiac Tissue

Jeremy A. Schaefer, Robert T Tranquillo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We developed a high-throughput screening assay that allows for relative comparison of the twitch force of millimeter-scale gel-based cardiac tissues. This assay is based on principles taken from traction force microscopy and uses fluorescent microspheres embedded in a soft polydimethylsiloxane (PDMS) substrate. A gel-forming cell suspension is simply pipetted onto the PDMS to form hemispherical cardiac tissue samples. Recordings of the fluorescent bead movement during tissue pacing are used to determine the maximum distance that the tissue can displace the elastic PDMS substrate. In this study, fibrin gel hemispheres containing human induced pluripotent stem cell-derived cardiomyocytes were formed on the PDMS and allowed to culture for 9 days. Bead displacement values were measured and compared to direct force measurements to validate the utility of the system. The amplitude of bead displacement correlated with direct force measurements, and the twitch force generated by the tissues was the same in 2 and 4 mg/mL fibrin gels, even though the 2 mg/mL samples visually appear more contractile if the assessment were made on free-floating samples. These results demonstrate the usefulness of this assay as a screening tool that allows for rapid sample preparation, data collection, and analysis in a simple and cost-effective platform.

Original languageEnglish (US)
Pages (from-to)76-83
Number of pages8
JournalTissue Engineering - Part C: Methods
Volume22
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Atomic Force Microscopy
Polydimethylsiloxane
Microscopic examination
Tissue
Gels
Assays
Force measurement
Fibrin
Screening
High-Throughput Screening Assays
Induced Pluripotent Stem Cells
Traction
Substrates
Stem cells
Microspheres
Cardiac Myocytes
Suspensions
Throughput
Costs and Cost Analysis
baysilon

Cite this

Tissue Contraction Force Microscopy for Optimization of Engineered Cardiac Tissue. / Schaefer, Jeremy A.; Tranquillo, Robert T.

In: Tissue Engineering - Part C: Methods, Vol. 22, No. 1, 01.01.2016, p. 76-83.

Research output: Contribution to journalArticle

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