Abstract
Aedes albopictus mosquito cells resistant to the thymidine analog 5- bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [3H]uridine and [3H]thymidine, and thymidine kinase activity in crude extracts. The LC50 for TK-6 cells was 95 μg BrdU/ml, and for TK-8 cells was 45 μg/ml. Both clones incorporated [3H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [3H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2 μM and a Vmax of 10 pmol · min-1 · mg-1 protein.
Original language | English (US) |
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Pages (from-to) | 455-458 |
Number of pages | 4 |
Journal | In Vitro Cellular and Developmental Biology - Animal |
Volume | 28 A |
Issue number | 6 |
DOIs | |
State | Published - Jan 1 1992 |