Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells

C. A. Mazzacano; A. M. Fallon

doi:10.1007/BF02634051

Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells

C. A. Mazzacano, A. M. Fallon

Entomology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Aedes albopictus mosquito cells resistant to the thymidine analog 5- bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [³H]uridine and [³H]thymidine, and thymidine kinase activity in crude extracts. The LC₅₀ for TK-6 cells was 95 μg BrdU/ml, and for TK-8 cells was 45 μg/ml. Both clones incorporated [³H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [³H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2 μM and a Vmax of 10 pmol · min^-1 · mg^-1 protein.

Original language	English (US)
Pages (from-to)	455-458
Number of pages	4
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	28 A
Issue number	6
DOIs	https://doi.org/10.1007/BF02634051
State	Published - Jan 1 1992

Access

10.1007/BF02634051

Fingerprint Dive into the research topics of 'Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells'. Together they form a unique fingerprint.

Cite this

@article{df603b47eb8b488db6b428a14950e53b,

title = "Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells",

abstract = "Aedes albopictus mosquito cells resistant to the thymidine analog 5- bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [3H]uridine and [3H]thymidine, and thymidine kinase activity in crude extracts. The LC50 for TK-6 cells was 95 μg BrdU/ml, and for TK-8 cells was 45 μg/ml. Both clones incorporated [3H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [3H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2 μM and a Vmax of 10 pmol · min-1 · mg-1 protein.",

author = "Mazzacano, {C. A.} and Fallon, {A. M.}",

year = "1992",

month = jan,

day = "1",

doi = "10.1007/BF02634051",

language = "English (US)",

volume = "28 A",

pages = "455--458",

journal = "In Vitro Cellular & Developmental Biology",

issn = "1054-5476",

publisher = "Springer New York",

number = "6",

}

TY - JOUR

T1 - Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells

AU - Mazzacano, C. A.

AU - Fallon, A. M.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Aedes albopictus mosquito cells resistant to the thymidine analog 5- bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [3H]uridine and [3H]thymidine, and thymidine kinase activity in crude extracts. The LC50 for TK-6 cells was 95 μg BrdU/ml, and for TK-8 cells was 45 μg/ml. Both clones incorporated [3H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [3H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2 μM and a Vmax of 10 pmol · min-1 · mg-1 protein.

AB - Aedes albopictus mosquito cells resistant to the thymidine analog 5- bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [3H]uridine and [3H]thymidine, and thymidine kinase activity in crude extracts. The LC50 for TK-6 cells was 95 μg BrdU/ml, and for TK-8 cells was 45 μg/ml. Both clones incorporated [3H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [3H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2 μM and a Vmax of 10 pmol · min-1 · mg-1 protein.

UR - http://www.scopus.com/inward/record.url?scp=0026661507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026661507&partnerID=8YFLogxK

U2 - 10.1007/BF02634051

DO - 10.1007/BF02634051

M3 - Article

C2 - 1634444

AN - SCOPUS:0026661507

VL - 28 A

SP - 455

EP - 458

JO - In Vitro Cellular & Developmental Biology

JF - In Vitro Cellular & Developmental Biology

SN - 1054-5476

IS - 6

ER -