Thymidine kinase-deficient mutants of Aedes albopictus mosquito cells

C. A. Mazzacano, A. M. Fallon

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5 Scopus citations

Abstract

Aedes albopictus mosquito cells resistant to the thymidine analog 5- bromodeoxyuridine (BrdU) were obtained using a single-step selection procedure. The resistant cells were characterized with respect to growth in the presence of BrdU, incorporation of [3H]uridine and [3H]thymidine, and thymidine kinase activity in crude extracts. The LC50 for TK-6 cells was 95 μg BrdU/ml, and for TK-8 cells was 45 μg/ml. Both clones incorporated [3H]uridine at levels corresponding to those in wild-type cells. TK-6 and TK-8 cells did not incorporate [3H]thymidine into acid-precipitable material, nor did they contain measurable thymidine kinase activity. Thymidine kinase activity in crude extracts from wild-type cells had a Km of 2 μM and a Vmax of 10 pmol · min-1 · mg-1 protein.

Original languageEnglish (US)
Pages (from-to)455-458
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume28 A
Issue number6
DOIs
StatePublished - Jan 1 1992

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