Thrombin activation of PAR-1 contributes to microvascular stasis in mouse models of sickle cell disease

Erica M. Sparkenbaugh, Chunsheng Chen, Tomasz Brzoska, Julia Nguyen, Shaobin Wang, Gregory M. Vercellotti, Nigel S. Key, Prithu Sundd, John D. Belcher, Rafal Pawlinski

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Vaso-occlusive crisis (VOC) is the primary cause of morbidity and hospitalization in sickle cell disease (SCD); however, only 4 therapies (hydroxyurea, L-glutamine, crizanlizumab, and voxeletor) are currently approved in SCD. These agents limit the duration, severity, and frequency of crises. Activation of coagulation is a hallmark of SCD. Studies in animalmodels of SCD have shown that coagulation contributes to the chronic inflammation and end-organ damage associated with the disease; however, it is unknown whether coagulation directly contributes to the microvascular stasis that causes VOC. Herein, we demonstrate that inhibition of tissue factor (TF) and the downstream coagulation proteases factor Xa and thrombin significantly attenuates heme-induced microvascular stasis in mouse models of VOC. Pharmacologic inhibition of the principal thrombin receptor, protease activated receptor-1 (PAR-1), as well as deficiency of PAR-1 in all nonhematopoietic cells, also reduces stasis in sicklemice. PAR-1 deficiency was associated with reduced endothelial von Willebrand factor expression, which has been shown to mediate microvascular stasis. In addition, TF inhibition reduces lung vaso-occlusion in sicklemicemediated by arteriolar neutrophil-plateletmicroemboli. In sum, these results suggest that prophylactic anticoagulation might attenuate the incidence of VOC.

Original languageEnglish (US)
Pages (from-to)1783-1787
Number of pages5
JournalBlood
Volume135
Issue number20
DOIs
StatePublished - May 14 2020

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health, National Heart, Lung, and Blood Institute grants R01 HL142604, R01 HL114567, R01 HL128297, R01 HL141080, and T32 HL007149.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

Fingerprint Dive into the research topics of 'Thrombin activation of PAR-1 contributes to microvascular stasis in mouse models of sickle cell disease'. Together they form a unique fingerprint.

Cite this