Three structural representatives of the PF06855 protein domain family from Staphyloccocus aureus and Bacillus subtilis have SAM domain-like folds and different functions

G. V.T. Swapna; Paolo Rossi; Alexander F. Montelione; Jordi Benach; Bomina Yu; Mariam Abashidze; Jayaraman Seetharaman; Rong Xiao; Thomas B. Acton; Liang Tong; Gaetano T. Montelione

doi:10.1007/s10969-012-9134-6

Three structural representatives of the PF06855 protein domain family from Staphyloccocus aureus and Bacillus subtilis have SAM domain-like folds and different functions

G. V.T. Swapna, Paolo Rossi, Alexander F. Montelione, Jordi Benach, Bomina Yu, Mariam Abashidze, Jayaraman Seetharaman, Rong Xiao, Thomas B. Acton, Liang Tong, Gaetano T. Montelione

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Protein domain family PF06855 (DUF1250) is a family of small domains of unknown function found only in bacteria, and mostly in the order Bacillales and Lactobacillales. Here we describe the solution NMR or X-ray crystal structures of three representatives of this domain family, MW0776 and MW1311 from Staphyloccocus aureus and yozE from Bacillus subtilis. All three proteins adopt a four-helix motif similar to sterile alpha motif (SAM) domains. Phylogenetic analysis classifies MW1311 and yozE as functionally equivalent proteins of the UPF0346 family of unknown function, but excludes MW0776, which likely has a different biological function. Our structural characterization of the three domains supports this separation of function. The structures of MW0776, MW1311, and yozE constitute the first structural representatives from this protein domain family.

Original language	English (US)
Pages (from-to)	163-170
Number of pages	8
Journal	Journal of Structural and Functional Genomics
Volume	13
Issue number	3
DOIs	https://doi.org/10.1007/s10969-012-9134-6
State	Published - Sep 2012
Externally published	Yes

Bibliographical note

Funding Information:
Schwarnof for access to beamline X4A at Brookhaven National Laboratory. The NESG expression vectors SR391-21.2, ZR218-21.1, and ZR215-21.1 have been deposited in the PSI Materials Repository (http://psimr.asu.edu/). This research was supported by the Protein Structure Initiative of the US National Institutes of Health, grants U54 GM074958 and U54 GM094597.

Keywords

DUF1250
MW0776
MW1311
PF06855
SAM domain
Solution NMR
Structural genomics
UPF0345
X-ray crystal structure
yozE

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Swapna, G. V. T., Rossi, P., Montelione, A. F., Benach, J., Yu, B., Abashidze, M., Seetharaman, J., Xiao, R., Acton, T. B., Tong, L., & Montelione, G. T. (2012). Three structural representatives of the PF06855 protein domain family from Staphyloccocus aureus and Bacillus subtilis have SAM domain-like folds and different functions. Journal of Structural and Functional Genomics, 13(3), 163-170. https://doi.org/10.1007/s10969-012-9134-6

Three structural representatives of the PF06855 protein domain family from Staphyloccocus aureus and Bacillus subtilis have SAM domain-like folds and different functions. / Swapna, G. V.T.; Rossi, Paolo; Montelione, Alexander F. et al.
In: Journal of Structural and Functional Genomics, Vol. 13, No. 3, 09.2012, p. 163-170.

Research output: Contribution to journal › Article › peer-review

Swapna, GVT, Rossi, P, Montelione, AF, Benach, J, Yu, B, Abashidze, M, Seetharaman, J, Xiao, R, Acton, TB, Tong, L & Montelione, GT 2012, 'Three structural representatives of the PF06855 protein domain family from Staphyloccocus aureus and Bacillus subtilis have SAM domain-like folds and different functions', Journal of Structural and Functional Genomics, vol. 13, no. 3, pp. 163-170. https://doi.org/10.1007/s10969-012-9134-6

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abstract = "Protein domain family PF06855 (DUF1250) is a family of small domains of unknown function found only in bacteria, and mostly in the order Bacillales and Lactobacillales. Here we describe the solution NMR or X-ray crystal structures of three representatives of this domain family, MW0776 and MW1311 from Staphyloccocus aureus and yozE from Bacillus subtilis. All three proteins adopt a four-helix motif similar to sterile alpha motif (SAM) domains. Phylogenetic analysis classifies MW1311 and yozE as functionally equivalent proteins of the UPF0346 family of unknown function, but excludes MW0776, which likely has a different biological function. Our structural characterization of the three domains supports this separation of function. The structures of MW0776, MW1311, and yozE constitute the first structural representatives from this protein domain family.",

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AU - Seetharaman, Jayaraman

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AU - Acton, Thomas B.

AU - Tong, Liang

AU - Montelione, Gaetano T.

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N2 - Protein domain family PF06855 (DUF1250) is a family of small domains of unknown function found only in bacteria, and mostly in the order Bacillales and Lactobacillales. Here we describe the solution NMR or X-ray crystal structures of three representatives of this domain family, MW0776 and MW1311 from Staphyloccocus aureus and yozE from Bacillus subtilis. All three proteins adopt a four-helix motif similar to sterile alpha motif (SAM) domains. Phylogenetic analysis classifies MW1311 and yozE as functionally equivalent proteins of the UPF0346 family of unknown function, but excludes MW0776, which likely has a different biological function. Our structural characterization of the three domains supports this separation of function. The structures of MW0776, MW1311, and yozE constitute the first structural representatives from this protein domain family.

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Three structural representatives of the PF06855 protein domain family from Staphyloccocus aureus and Bacillus subtilis have SAM domain-like folds and different functions

Abstract

Bibliographical note

Keywords

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