Three Small Ribooligonucleotides with Specific Arginine Sites

Gregory J. Connell, Mali Illangesekare, Michael Yarus

Research output: Contribution to journalArticlepeer-review

177 Scopus citations


Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an l-arginine affinity column (Kd ≈ 0.2–0.4 mM). The binding sites contain specific internal and bulge loops, whose sequences can include arginine coding triplets. Binding is highly specific for arginine, but all three motifs, like the self-splicing group I intron, also bind guanosine 5′-monophosphate. One site is stereoselective, somewhat preferring d-arginine.

Original languageEnglish (US)
Pages (from-to)5497-5502
Number of pages6
Issue number21
StatePublished - 1993


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