Three-dimensional imaging of intact isolated islets of Langerhans with confocal microscopy

T. C. Brelje, D. W. Scharp, Robert L Sorenson

Research output: Contribution to journalArticlepeer-review

89 Scopus citations


We present a new technique for analyzing the three-dimensional structure of intact isolated islets of Langerhans. Adult rat and human islets were stained with whole-mount immunofluorescence techniques and optically sectioned with a confocal microscope. This has several advantages over traditional methods: 1) the technical difficulties in serial sectioning and handling the large numbers of sections are avoided, 2) optical sectioning by confocal microscopy gives improved resolution and strongly suppresses light from out-of-focus structures, and 3) entire islets can be rapidly imaged for the presence of positive staining. This new technique should facilitate the study of the three-dimensional structure of islets of Langerhans.

Original languageEnglish (US)
Pages (from-to)808-814
Number of pages7
Issue number6
StatePublished - 1989


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