Three-dimensional architecture of the bacteriophage φ29 packaged genome and elucidation of its packaging process

Luis R. Comolli, Andrew J. Spakowitz, Cristina E. Siegerist, Paul J. Jardine, Shelley Grimes, Dwight L. Anderson, Carlos Bustamante, Kenneth H. Downing

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

The goal of the work reported here is to understand the precise molecular mechanism of the process of DNA packaging in dsDNA bacteriophages. Cryo-EM was used to directly visualize the architecture of the DNA inside the capsid and thus to measure fundamental physical parameters such as inter-strand distances, local curvatures, and the degree of order. We obtained cryo-EM images of bacteriophage that had packaged defined fragments of the genome as well as particles that had partially completed the packaging process. The resulting comparison of structures observed at intermediate and final stages shows that there is no unique, deterministic DNA packaging pathway. Monte Carlo simulations of the packaging process provide insights on the forces involved and the resultant structures.

Original languageEnglish (US)
Pages (from-to)267-277
Number of pages11
JournalVirology
Volume371
Issue number2
DOIs
StatePublished - Feb 20 2008

Keywords

  • Bacteriophage structure
  • Cryo-electron microscopy
  • DNA packaging
  • Monte Carlo
  • φ29

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    Comolli, L. R., Spakowitz, A. J., Siegerist, C. E., Jardine, P. J., Grimes, S., Anderson, D. L., Bustamante, C., & Downing, K. H. (2008). Three-dimensional architecture of the bacteriophage φ29 packaged genome and elucidation of its packaging process. Virology, 371(2), 267-277. https://doi.org/10.1016/j.virol.2007.07.035