Thiostrepton-resistant mutants of Bacillus subtilis: Localization of resistance to the 50S subunit

Sidney Pestka, David Weiss, Robert Vince, Bertold Wienen, Georg Stöffler, Issar Smith

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16 Scopus citations


A number of thiostrepton-resistant mutants of Bacillus subtilis were obtained. The thi mutations map proximally to strA. Effects of thiostrepton on polyphenylalanine synthesis with ribosomes and S-100 fractions from parent and mutant strains indicated that resistance was localized to the ribosomes. Furthermore, effects of thiostrepton on binding of [3H]GTP to ribosomes and 50S subunits from thiostrepton-sensitive and-resistant strains localized the site of resistance to the 50S subunit. In addition, revertants from thiostrepton-resistance to thiostrepton-sensitivity were obtained. Ribosomes and 50S subunits from these thiostrepton-sensitive revertants were sensitive to thiostrepton similar to parental sensitive B. subtilis.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalMGG Molecular & General Genetics
Issue number3
StatePublished - Jan 1976
Externally publishedYes


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