Thiolate Ligation of the Active Site Fe2+of Isopenicillin N Synthase Derives from Substrate Rather Than Endogenous Cysteine: Spectroscopic Studies of Site-Specific Cys Â-Ser Mutated Enzymes

Allen M. Orville, Victor J. Chen, Aidas Kriauciunas, Mark R. Harpel, Brian G. Fox, Eckard Münck, John D. Lipscomb

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Medicine & Life Sciences

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