THERMOMETRIC INVESTIGATION OF THE REACTION BETWEEN PROTEINS AND 12-PHOSPHOTUNGSTIC ACID.

P. W. Carr, E. B. Smith, S. R. Betson, R. H. Callicott

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

In the reported investigation, precipitation of proteins in solution of 12-phosphotungstic acid was studied by using thermometric titration technique. In all of the titrations reported the protein was in the concentration range of 2-5 g/l and the titrant was added at a rate of 1. 26 mu equivalent/sec. Photometric measurements of protein concentration were carried out on a Cary 14 recording spectrophotometer. Amperometric measurements were carried out with a Heath polarographic unit. A set of thermometric titration curves of bovine albumin with PTA at acidities ranging from pH 2 to 5 are shown. Most titrations reported here were carried out at pH 1. In general the data correlate quite well with the sum of basic amino acids in the protein. The thermometric titration curves indicate that the first stage of reaction is more exothermic than the second. This may be rationalized by assuming that chloride binding to proteins is exothermic and that the heat of binding PTA to a given site is independent of whether the neighboring sites are vacant or occupied by chloride.

Original languageEnglish (US)
Pages457-464
Number of pages8
StatePublished - Jan 1 1974
EventAm Chem Soc Symp on Anal Calorimetry, 3rd, Proc - Los Angeles, CA, USA
Duration: Mar 30 1974Apr 5 1974

Other

OtherAm Chem Soc Symp on Anal Calorimetry, 3rd, Proc
CityLos Angeles, CA, USA
Period3/30/744/5/74

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Titration
Proteins
Acids
Spectrophotometers
Acidity
Amino acids

Cite this

Carr, P. W., Smith, E. B., Betson, S. R., & Callicott, R. H. (1974). THERMOMETRIC INVESTIGATION OF THE REACTION BETWEEN PROTEINS AND 12-PHOSPHOTUNGSTIC ACID.. 457-464. Paper presented at Am Chem Soc Symp on Anal Calorimetry, 3rd, Proc, Los Angeles, CA, USA, .

THERMOMETRIC INVESTIGATION OF THE REACTION BETWEEN PROTEINS AND 12-PHOSPHOTUNGSTIC ACID. / Carr, P. W.; Smith, E. B.; Betson, S. R.; Callicott, R. H.

1974. 457-464 Paper presented at Am Chem Soc Symp on Anal Calorimetry, 3rd, Proc, Los Angeles, CA, USA, .

Research output: Contribution to conferencePaper

Carr, PW, Smith, EB, Betson, SR & Callicott, RH 1974, 'THERMOMETRIC INVESTIGATION OF THE REACTION BETWEEN PROTEINS AND 12-PHOSPHOTUNGSTIC ACID.' Paper presented at Am Chem Soc Symp on Anal Calorimetry, 3rd, Proc, Los Angeles, CA, USA, 3/30/74 - 4/5/74, pp. 457-464.
Carr PW, Smith EB, Betson SR, Callicott RH. THERMOMETRIC INVESTIGATION OF THE REACTION BETWEEN PROTEINS AND 12-PHOSPHOTUNGSTIC ACID.. 1974. Paper presented at Am Chem Soc Symp on Anal Calorimetry, 3rd, Proc, Los Angeles, CA, USA, .
Carr, P. W. ; Smith, E. B. ; Betson, S. R. ; Callicott, R. H. / THERMOMETRIC INVESTIGATION OF THE REACTION BETWEEN PROTEINS AND 12-PHOSPHOTUNGSTIC ACID. Paper presented at Am Chem Soc Symp on Anal Calorimetry, 3rd, Proc, Los Angeles, CA, USA, .8 p.
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