TY - JOUR
T1 - Thermal stability of plasminogen activators and plasminogen activation in heated milk
AU - Prado, B. M.
AU - Ismail, B.
AU - Ramos, O.
AU - Hayes, K. D.
PY - 2007/9
Y1 - 2007/9
N2 - Thermal stabilities of bovine plasminogen (PG) activators, tissue and urokinase type (t-PA and u-PA), and their impact on activation of PG, were studied after application of various heating conditions (65, 75, 85, or 90 °C for 15, 20, or 30 s), in both milk and buffer systems. Both t-PA and u-PA were thermally stable in milk heated at ≤75 °C. However, almost half of the t-PA activity and 30% of u-PA activity was lost after heating milk at 85 °C for 30 s. A lower heat stability of both t-PA and u-PA was observed in buffer than in milk. The decrease in level of PG was more pronounced than that of active plasmin in milk heated at ≥85 °C for 30 s; however, the residual level of PG was considerably higher than the residual level of active PL. Overall, results indicated that u-PA plays a more significant role than t-PA in the activation of PG during storage of heated milk.
AB - Thermal stabilities of bovine plasminogen (PG) activators, tissue and urokinase type (t-PA and u-PA), and their impact on activation of PG, were studied after application of various heating conditions (65, 75, 85, or 90 °C for 15, 20, or 30 s), in both milk and buffer systems. Both t-PA and u-PA were thermally stable in milk heated at ≤75 °C. However, almost half of the t-PA activity and 30% of u-PA activity was lost after heating milk at 85 °C for 30 s. A lower heat stability of both t-PA and u-PA was observed in buffer than in milk. The decrease in level of PG was more pronounced than that of active plasmin in milk heated at ≥85 °C for 30 s; however, the residual level of PG was considerably higher than the residual level of active PL. Overall, results indicated that u-PA plays a more significant role than t-PA in the activation of PG during storage of heated milk.
KW - Heat treatment
KW - Plasminogen
KW - Plasminogen activator
KW - Tissue
KW - Urokinase
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U2 - 10.1016/j.idairyj.2007.02.001
DO - 10.1016/j.idairyj.2007.02.001
M3 - Article
AN - SCOPUS:34249978928
SN - 0958-6946
VL - 17
SP - 1028
EP - 1033
JO - International Dairy Journal
JF - International Dairy Journal
IS - 9
ER -