Therapeutic interventions using a combination of Telmisartan and omega 3-fatty acids in sodium arsenite-induced vascular endothelial dysfunction in rats: Modulation through ATP-sensitive K+ channels and eNOS

Abhijit Nirwane, Vivek Pawar, Anuradha Majumdar

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background: Effective diet/drug combinations may show additive or synergistic effects in reducing endothelial risk factors vis-à-vis monotherapies. The study evaluated the effect of combined therapy of Telmisartan and omega 3-fatty acids in sodium arsenite-induced vascular endothelial dysfunction (VED) in rats. Methods: Forty-eight male Wistar rats (180-220 g) were randomized into eight groups; control, sodium arsenite (1.5 mg/kg/day) exposed, sodium arsenite exposure followed by treatment with Telmisartan, omega 3-fatty acids, the combination and/or endothelial modulators for 2 weeks depending on the allocated group. VED was assessed by estimating vascular reactivity. Serum thiobarbituric acid-reactive substances (TBARS), nitrite/nitrate levels, reduced glutathione (GSH) levels, superoxide dismutase (SOD) activity, serum cholesterol and triglyceride levels were also determined. Results: Sodium arsenite produced VED by attenuating acetylcholine-induced endothelial relaxation (% Rmax= 45.36), decreasing levels of serum nitrite/nitrate (9.28 μM/mg protein), GSH (16.06 μg/mg of protein), SOD activity (30.69 units/mg protein) and increasing TBARS (0.19 μM/mg protein) compared with control group. The combined therapy with Telmisartan (10 mg/kg/day) and omega 3-fatty acids (180 mg/kg/day) (% Rmax=80.93, 13.09 μM/mg protein, 25.93 μg/mg of protein, 57.84 units/mg protein and 0.08 μM/mg protein, respectively) significantly abolished the respective derangements induced by sodium arsenite. Further, this combination significantly prevented rise in serum cholesterol and triglyceride levels that was induced by sodium arsenite. However, the ameliorative effects of this combination were abated by N-omega-nitro-L-arginine methyl ester (L-NAME) and glibenclamide. Conclusions: Combined therapy of Telmisartan and omega 3-fatty acids attenuated VED, by activating enzyme nitric oxide synthase (eNOS) through opening of ATP-sensitive K+ channels.

Original languageEnglish (US)
Pages (from-to)143-151
Number of pages9
JournalJournal of Complementary and Integrative Medicine
Volume12
Issue number2
DOIs
StatePublished - Jun 1 2015

Keywords

  • Telmisartan
  • diet/drug therapy
  • nitric oxide
  • omega 3-fatty acids
  • vascular endothelial dysfunction

Fingerprint Dive into the research topics of 'Therapeutic interventions using a combination of Telmisartan and omega 3-fatty acids in sodium arsenite-induced vascular endothelial dysfunction in rats: Modulation through ATP-sensitive K<sup>+</sup> channels and eNOS'. Together they form a unique fingerprint.

Cite this