Therapeutic doses of radiation alter proliferation and attachment of osteoblasts to implant surfaces

Mansur Ahmad, Christopher Sampair, Abu N.M. Nazmul-Hossain, Neerja Khurana, Andrew Nerness, Patcharaporn Wutticharoenmongkol

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Osseointegration of implants in irradiated bone is inadequate. The effect of radiation on cell-implant material interaction has not been adequately studied. The goal of this study was to investigate the effects of ionizing radiation on the proliferation, differentiation, and attachment of osteoblasts to commercially pure titanium (cpTi). Human fetal osteoblasts (hFOB) were irradiated either before or after plating in tissue culture (TC) dishes with or without cpTi disks. Radiation was single dose of 10 cGy, 25 cGy, 50 cGy, 1 Gy, 2 Gy, 4 Gy or 8 Gy. Cell proliferation was determined by counting trypsinized cells on 7 days after irradiation. Attachment of irradiated hFOB was measured indirectly by counting cells 2 and 6 h after plating. Differentiation was evaluated by alkaline phosphatase activity. Compared with nonirradiated sham controls, higher doses of radiation significantly reduced cell attachment and proliferation. Both proliferation and attachment were significantly lower on cpTi compared with TC. Attachment decreased based on the length of postirradiation period. Although differentiation was significantly enhanced by a dose of 8 Gy, proliferation was lowest. These initial studies show that effects of therapeutic doses of radiation on osteoblasts varied depending on the surface, time-elapsed, and amount of radiation.

Original languageEnglish (US)
Pages (from-to)926-934
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Issue number4
StatePublished - Sep 15 2008


  • Attachment
  • Ionizing radiation
  • Osteoblasts
  • Proliferation
  • Titanium


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