Theileria parva: Early events in the development of bovine lymphoblastoid cell lines persistently infected with macroschizonts

T. J. Kurtti, U. G. Munderloh, A. D. Irvin, G. Büscher

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24 Scopus citations

Abstract

The cellular origin and development of bovine lymphoblastoid cell lines persistently infected with macroschizonts of Theileria parva was studied. Cultures of lymphoblastoid cells isolated from cattle with patent East Coast fever were compared with those obtained by infecting normal lymphocytes in vitro with sporozoites. The young lines were contrasted with a continuous line which had been isolated earlier. The mononuclear cells were separated from the blood and the inoculum enriched for lymphoblastoid cells and/or lymphocytes by removing the monocytes. The lines arose directly from lymphoblastoid cells transplanted into culture or from lymphocytes infected by sporozoites. In primary cultures of lymphoblastoid cells from the peripheral blood, there was an increase in the proportion of infected cells without the eclipse of the parasite, the macroschizonts were larger than those observed in the inoculum or the continuous line, and there was concurrent microschizont differentiation. In lymphocyte cultures challenged with sporozoites, small mononucleated trophozoites were observed after 2 days which differentiated into typical macroschizonts but microschizonts were rare. In all cultures, the infected cells had mitotic indices of 4 to 5%. As the young lines were passaged, the parasites came to resemble those of the continuous line. The macroschizont size in the continuous line was stable and most had six to eight nuclei but when cultured at high cell concentrations the number of parasite nuclei increased. Minicultures of lymphocytes were used to quantitate the infectivity of sporozoites obtained from organ cultures of Rhipicephalus appendiculatus savliary glands. Sporozoites from ticks fed on rabbits for 5 days were approximately six times more infective than those from glands of ticks fed for 2 days and then cultured at 32 °C for 3 days. Glands from unfed ticks cultured for 5 days failed to yield infective sporozoites.

Original languageEnglish (US)
Pages (from-to)280-290
Number of pages11
JournalExperimental Parasitology
Volume52
Issue number2
DOIs
StatePublished - Oct 1981

Keywords

  • Bovine host
  • East Coast fever
  • Lymphoblastoid cell lines
  • Macroschizonts
  • Protozoa, parasitic
  • Rhipicephalus appendiculatus
  • Salivary gland organ cultures
  • Sporozoites
  • Theileria parva
  • Tick

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