The Zygotic Transition Is Initiated in Unicellular Plant Zygotes with Asymmetric Activation of Parental Genomes

Sarah N. Anderson, Cameron S. Johnson, Joshua Chesnut, Daniel S. Jones, Imtiyaz Khanday, Margaret Woodhouse, Chenxin Li, Liza J. Conrad, Scott D. Russell, Venkatesan Sundaresan

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The zygotic transition, from a fertilized egg to an embryo, is central to animal and plant reproduction. Animal embryos depend upon maternally provided factors until zygotic genome activation (ZGA). In plants, the timing and parental genome contributions to ZGA are unresolved. Here, we use the flowering plant Oryza sativa (rice) to characterize transcriptomes of time-staged isogenic and hybrid zygotes following fertilization. Large-scale transcriptomic changes were observed in unicellular zygotes, including upregulation of S-phase genes, a characteristic of ZGA. The parental contributions to ZGA were highly asymmetric. Zygotic transcription was primarily from the maternal genome and included genes for basic cellular processes. Transcription of the paternal genome was highly restricted but unexpectedly included genes encoding putative pluripotency factors expressed at the onset of ZGA. Thus, distinct transcriptional activities are exhibited by the parental genomes during the initiation of embryogenesis, which presumptively derive from divergent pre-zygotic transcriptional states established in the gametes. The transition from maternal to zygotic gene expression is critical to multicellular organism development. Anderson et al. elucidate timing and parental genome contribution to zygotic genome activation (ZGA) in rice. ZGA occurs in the unicellular zygote, with unequal parental contribution where most genes are expressed primarily from the maternal genome.

Original languageEnglish (US)
Pages (from-to)349-358.e4
JournalDevelopmental Cell
Volume43
Issue number3
DOIs
StatePublished - Nov 6 2017

Bibliographical note

Funding Information:
We thank Eugene Lurie for technical assistance. This research was supported by research grants IOS-1128145 and IOS-1547760 from the National Science Foundation (NSF) and by the USDA Agricultural Experiment Station (project no. CA-D-XXX-6973-H ), and utilized the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 Instrumentation Grants S10RR029668 , S10RR027303 , and S10OD018174 .

Keywords

  • fertilization
  • genome activation
  • maternal to zygotic transition
  • plant development
  • plant reproduction

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