The yeast MSH1 gene is not involved in DNA repair or recombination during meiosis

Elaine A. Sia, David T Kirkpatrick

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Six strong homologs of the bacterial MutS DNA mismatch repair (MMR) gene have been identified in the yeast Saccharomyces cerevisiae. With the exception of the MSH1 gene, the involvement of each homolog in DNA repair and recombination during meiosis has been determined previously. Five of the homologs have been demonstrated to act in meiotic DNA repair (MSH2, MSH3, MSH6 and MSH4) and/or meiotic recombination (MSH4 and MSH5). Unfortunately the loss of mitochondrial function that results from deletion of MSH1 disrupts meiotic progression, precluding an analysis of MSH1 function in meiotic DNA repair and recombination. However, the recent identification of two separation-of-function alleles of MSH1 that interfere with protein function but still maintain functional mitochondria allow the meiotic activities of MSH1 to be determined. We show that the G776D and F105A alleles of MSH1 exhibit no defects in meiotic recombination, repair base-base mismatches and large loop mismatches efficiently during meiosis, and have high levels of spore viability. These data indicate that the MSH1 protein, unlike other MutS homologs in yeast, plays no role in DNA repair or recombination during meiosis.

Original languageEnglish (US)
Pages (from-to)253-261
Number of pages9
JournalDNA Repair
Volume4
Issue number2
DOIs
StatePublished - Feb 3 2005

Fingerprint

Meiosis
DNA Repair
Yeast
Genetic Recombination
Repair
Genes
Yeasts
DNA
Alleles
Recombinational DNA Repair
Bacterial DNA
DNA Mismatch Repair
Spores
Saccharomyces cerevisiae
Mitochondria
Proteins
Defects

Keywords

  • MSH1
  • Meiosis
  • Mismatch repair
  • Mitochondria
  • MutS
  • Recombination

Cite this

The yeast MSH1 gene is not involved in DNA repair or recombination during meiosis. / Sia, Elaine A.; Kirkpatrick, David T.

In: DNA Repair, Vol. 4, No. 2, 03.02.2005, p. 253-261.

Research output: Contribution to journalArticle

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