The winged-helix/forkhead protein myocyte nuclear factor β (MNF-β) forms a co-repressor complex with mammalian Sin3B

Quan Yang, Yanfeng Kong, Beverly Rothermel, Daniel J. Garry, Rhonda Bassel-Duby, R. Sanders Williams

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Winged-helix/forkhead proteins regulate developmental events in both invertebrate and vertebrate organisms, but biochemical functions that establish a mechanism of action have been defined for only a few members of this extensive gene family. Here we demonstrate that MNF (myocyte nuclear factor)-β, a winged-helix protein expressed selectively and transiently in myogenic precursor cells of the heart and skeletal muscles, collaborates with proteins of the mammalian Sin3 (mSin3) family to repress transcription. Mutated forms of MNF-β that fail to bind mSin3 are defective in transcriptional repression and in negative growth regulation, an overexpression phenotype revealed in oncogenic transformation assays. These data extend the known repertoire of transcription factors with which mSin3 proteins can function as co-repressors to include members of the winged-helix gene family. Transcriptional repression by MNF-β-mSin3 complexes may contribute to the co-ordination of cellular proliferation and terminal differentiation of myogenic precursor cells.

Original languageEnglish (US)
Pages (from-to)335-343
Number of pages9
JournalBiochemical Journal
Volume345
Issue number2
DOIs
StatePublished - Jan 15 2000

Keywords

  • Myogenic cells
  • Protein interactions
  • Repression
  • Transcription

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