The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley

M. Asyraf Md Hatta, Sanu Arora, Sreya Ghosh, Oadi Matny, Mark A. Smedley, Guotai Yu, Soma Chakraborty, Dhara Bhatt, Xiaodi Xia, Burkhard Steuernagel, Terese Richardson, Rohit Mago, Evans S. Lagudah, Nicola J. Patron, Michael Ayliffe, Matthew N. Rouse, Wendy A. Harwood, Sambasivam Periyannan, Brian J. Steffenson, Brande B.H. Wulff

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley’s genetic variation for stem rust resistance is very narrow with only ten resistance genes genetically identified. Of these, only one complex locus consisting of three genes is effective against TTKSK, a widely virulent Pgt race of the Ug99 tribe which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against race TTKSK. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium-mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Furthermore, these transgenic plants did not display significant adverse agronomic effects in the absence of disease. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley.

Original languageEnglish (US)
Pages (from-to)273-284
Number of pages12
JournalPlant Biotechnology Journal
Volume19
Issue number2
DOIs
StatePublished - Feb 2021

Bibliographical note

Funding Information:
The authors wish to thank the support of the Two Blades Foundation, USA, the Lieberman-Okinow Endowment at the University of Minnesota, the USDA-ARS, and the Biotechnology and Biological Sciences Research Council, UK, Grant number BB/L011794/1 and the cross-institute strategic programme Designing Future Wheat (BB/P016855/1). SG was supported by a Monsanto?s Beachell-Borlaug International Scholars Program fellowship (06-400258-12580) and John Innes Centre International Scholarship Scheme. MAMdH was supported by a fellowship from Universiti Putra Malaysia (UPM), Malaysia. We thank Eduard Akhunov, Kansas State University, for supplying seed of Sr35 T.?monococcum. We also thank Ryan Johnson, University of Minnesota, for carrying out phenotyping of the studied lines.

Keywords

  • Sr22
  • Sr33
  • Sr35
  • Sr45
  • Ug99
  • barley
  • durable disease resistance
  • stacking
  • stem rust
  • wheat

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