The wag31gene of Mycobacterium tuberculosisis positively regulated by the stringent response

John L. Dahl, Dalia Lau Bonilla

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The stringent response of Mycobacterium tuberculosis is coordinated by Rel and is required for full virulence in animal models. A serological-based approach identified Wag31Mtb as a protein that is upregulated in M. tuberculosis in a rel-dependent manner. This positive regulation was confirmed by analysis of M. tuberculosis mRNA expression. Mycobacterium smegmatis was used to confirm that the expression of wag31Mtb from its native promoter is positively regulated by the stringent response. Furthermore, elevated wag31Mtb expression in M. smegmatis drastically alters the cell-surface hydrophobic properties.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalFEMS Microbiology Letters
Volume319
Issue number2
DOIs
StatePublished - Jun 2011

Keywords

  • Mycobacterium smegmatis
  • Mycobacterium tuberculosis
  • Stringent response
  • rel
  • wag31

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